湿热型溃疡性结肠炎大鼠模型的建立与研究  

Model of Ulcerative Colitis Rats with Damp-heat Syndrome

作　　者：翁一洁[1] 郑学宝[1] 

机构地区：[1]广东医学院,广东湛江524023

出　　处：《时珍国医国药》2011年第10期2522-2525,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30772701);广东省中医药局建设中医药强省科研课题(No.2007011)

摘　　要：目的构建与人体最接近的湿热型溃疡性结肠炎大鼠模型,并探讨该模型的客观指标评价体系。方法根据前期研究的方法即结合内因湿热和免疫复合法诱发湿热型溃疡性结肠炎,观察大鼠证候学、结肠病理学及免疫学指标的改变,判定造模是否成功。结果模型组大鼠出现嗜睡懒动、蜷缩,饮少纳呆,精神萎靡,毛发疏松粗糙、晦暗无光泽,腹泻及血便明显。DAI、病理学评分、MTL、GAS、COX-2、IFN-γ、VCAM-1均升高(P<0.05或P<0.01)。结论采用高脂高糖辛辣饮食加免疫复合法可以构建出理想的湿热性溃疡性结肠炎大鼠模型。ObjectiveTo build the model of ulcerative colitis rat with damp-heat syndrome,and explore the evaluation system. MethodsThe model of ulcerative colitis rats with damp-heat syndrome was established and the effect was determined by rat Syndrome,colon pathology and immunological parameters. ResultsSleepiness,lazy move,curl up,drinkless,poor appetite,listlessness,rough hair loose,obvious diarrhea and bloody stool appeared in the model rats.DAI,pathology score,MTL,GAS,COX-2,IFN-γ,VCAM-1 were higher in model group than in normal control group（P0.05 or P0.01）. ConclusionSpicy food with high fat and sugar,plus immune complex method can build the ideal ulcerative colitis rat model with damp-heat syndrome.

关 键 词：湿热证 溃疡性结肠炎 动物模型 

分 类 号：R284.2[医药卫生—中药学]