小补心汤总黄酮含药血清对皮质酮损伤神经细胞的保护作用  

Protection of serum containing total flavonoids extracted from Xiaobuxin-Tang on corticosterone induced nerve injury

作　　者：李静[1,2] 田燕[2] 陈红霞[1] 于能江[1] 林原 张有志[1] 安磊[1,2] 李云峰[1] 

机构地区：[1]军事医学科学院毒物药物研究所全军军事认知与心理卫生研究中心,北京100850 [2]大连医科大学药学院,辽宁大连116044

出　　处：《军事医学》2011年第10期765-768,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(3090197530400600;81001653;81072624);国家973计划资助项目(2007CB512307)

摘　　要：目的探讨小补心汤总黄酮(XBXT-2)含药血清的神经保护作用。方法采用血清药理学方法分别制备大鼠的空白血清、氟西汀(10 mg/kg)和XBXT-2(25,50,100,200 mg/kg)含药血清。采用皮质酮损伤大鼠肾上腺嗜铬细胞瘤细胞(PC12)、人神经母细胞瘤细胞(SH-SY5Y)及原代培养大鼠海马神经元为体外药理学模型,应用ATP生物发光法检测XBXT-2及氟西汀含药血清对损伤细胞存活率的影响。结果皮质酮(50～300μmol/L)能够浓度依赖性地抑制SH-SY5Y的存活,氟西汀与XBXT-2(25,50 mg/kg)含药血清能显著提高皮质酮(100μmol/L)损伤的SH-SY5Y的存活率(P<0.01,P<0.05,P<0.01);与空白对照组比较,200μmol/L皮质酮导致PC12的存活率显著下降(P<0.01),氟西汀与XBXT-2(25,50,100 mg/kg)含药血清能显著对抗这种损伤(P<0.05,P<0.01,P<0.05);100μmol/L皮质酮使原代海马神经元存活率显著下降(P<0.001),XBXT-2(25、50 mg/kg)对这种损伤具有显著的保护作用(P<0.05,P<0.001),其作用与阳性药氟西汀含药血清相当。结论 XBXT-2含药血清对皮质酮所致的神经细胞损伤具有明显保护作用,这可能是其抗抑郁效应的重要细胞机制之一。ObjectiveTo investigate the neuroprotective effect of serum containing the total flavonoids extracted from Xiaobuxin-Tang（XBXT-2）.MethodsSerum pharmacology experiments were used to obtain rat serum including control serum,and serum containing fluoxetine（Flu,10 mg/kg） or XBXT-2（25,50,100 and 200 mg/kg）,respectively.Cultured PC12 cells,SH-SY5Y cells and primary culture of hippocampal neurons from neonatal rats were incubated with corticosterone（Cort） for 48 h in the presence of control serum or serum containing fluoxetine or XBXT-2.The cell viability was measured by ATP-luminescent cell viability assay.ResultsAfter incubation with Cort（50-300 μmol/L）,the cell viability of SH-SY5Y significantly decreased in a dose-dependent manner.Flu and XBXT-2（25 and 50 mg/kg） serum could significantly increase the cell viability of SH-SY5Y cells with Cort（100 μmol/L） co-incubation（P0.01,P0.05 and P0.01）.Compared with the control group,the cell viability of PC12 exposure to Cort（200 μmol/L） was significantly decreased（P0.01）,while the injury was significantly eased by serum containing Flu and XBXT-2（25,50 and 100 mg/kg）（P0.05,P0.01 and P0.05）.XBXT-2（25 and 50 mg/kg） could protect primary culture of hippocampal neurons from the lesion induced by Cort（100 μmol/L）（P0.05 and P0.001）,and the positive control fluoxetine（10 mg/kg） serum exerted the same effect.ConclusionSerum containing XBXT-2 shows significant protection against nerve injury induced by Cort,which may be one of the important cellular mechanisms underlying the antidepressant action of XBXT-2.

关 键 词：小补心汤 黄酮类 血清药理 神经保护 抑郁 

分 类 号：R285.5[医药卫生—中药学]