混合表面活性剂分光光度法测定痕量锌  

MIXED SURFATANT SPECTROPHOTOMETRIC OF TRACE ZINC

作　　者：崔新宇[1] 侯维志[2] 靳革[1] 王栋[1] 

机构地区：[1]牡丹江医学院公共卫生学院,黑龙江牡丹江157011 [2]牡丹江医学院学报编辑部,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2011年第5期7-9,共3页Journal of Mudanjiang Medical University

摘　　要：目的:在曲拉通-100(TritonX-100)和乳化剂(OP)存在下,基于Zn(Ⅱ)与2-(5-溴吡啶-2-偶氮)-5-二乙氨基苯酚(5-Br-PADAP)溶液的显色反应,建立了分光光度法测定痕量锌的新方法,同时为反应机理做一些基础实验。方法:通过添加TritonX-100-OP进入Zn(Ⅱ)-5-Br-PADAP体系提高灵敏度来测量锌含量。结果:体系最大吸收波长为575nm,表观摩尔吸光系数为ε=1.90×105 L.mol-1.cm-1,Zn(Ⅱ)含量在0.026～12.0μg/25mL范围内服从比尔定律,线性回归方程为A=0.3365C(μg/25mL)+0.1006r,=0.9992,干扰离子较少。结论:已用于人发中锌含量的测定,结果令人满意。Objective:A new color reaction method for the determination of trace zinc with 2-(5-bromo-pyridine-2-azobis) -5-diethylaminophenol(5-Br-PADAP) in the presence of TritonX-100 and emulsifier(OP) has been described.Methods: Zn(Ⅱ) with the 5-Br-PADAP-TritonX-100-OP to form a high sensitivity of multi complexes to measure the zinc content.Conclusion:The absorption maxima of the complex is at 575 nm with apparent molar absorptivity of 1.90 × 105 L · mol-1 · cm-1.The linear Range is 0.026～12.0 μg /25 mL.The regression equation is A=0.3365C(μg/25mL)+0.1006,the correlation coefficient is r= 0.9992,interfering ion less.Results:The method has been applied to the determination of trace zinc in hair with satisfactory results.

关 键 词：锌 分光光度法 2-(5-溴吡啶-2-偶氮)-5-二乙氨基苯酚 曲拉通-100 乳化剂 

分 类 号：O657.3[理学—分析化学]