芦花鸡传染性肿瘤病的流行病学调查  

Investigation of contagious tumor diseases in Luhua chicken

作　　者：王健[1] 杨凤[1] 孙洪磊[1] 倪伟[1] 车国喜[1] 刘思当[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2011年第11期1573-1577,共5页Chinese Journal of Veterinary Science

基　　金：农业部公益性行业科研专项资助项目(200803019)

摘　　要：从山东某养殖场散养的60日龄芦花鸡随机抽取100只隔离饲养,分别于65、120、190、240日龄翅静脉采血,动态检测禽白血病(AL)AB、J亚型抗体、网状内皮组织增殖病(RE)抗体;取泄殖腔棉拭子动态检测禽白血病病毒(ALV)群特异性抗原p27;同时对患瘤病死鸡进行剖检、病理组织学检查、PCR病原检测。结果显示,鸡群在240日龄时出现13.24%的ALV-AB抗体阳性率,未检测到ALV-J抗体,网状内皮组织增殖病病毒(REV)抗体在120日龄时出现阳性,且阳性率随日龄增加而升高;p27阳性率为34.21%～66.18%;开产前后鸡群开始出现肿瘤性疾病,一直持续至240日龄,死亡率达32%,120～190日龄为死亡高峰期;剖检病鸡可见内脏器官普遍肿大,肝、脾、肾表面及切面有大小不等的灰白色肿瘤样结节,部分病鸡坐骨神经肿胀;组织学检查,肿瘤组织为浸润性生长的多形态淋巴样细胞构成,符合马立克氏病(MD)的病变特征;PCR检测16份肿瘤病料中有14份为ALV-J阳性(87.5%),13份马立克氏病病毒(MDV)阳性(81.25%),2份为REV阳性(12.5%),ALV-J与MDV、REV混合感染率分别为68.8%(11/16)、12.5%(2/16),三重感染率为6.3%(1/16)。该芦花鸡群存在ALV、MDV和REV的共感染。To investigate the co-infection status of tumor diseases from Chinese native breed ＂Luhua＂ in Shandong province,serums were dynamically detected for antibody of ALV-J, ALV-A/B, REV, and antigen p27 of ALV from cloacal swabs samples of free range Luhua chickens separately at the age of 65,120,190 day and 240 day were also detected. The sick or dead chickens manifesting tumor lesions were necropsy ,histopathology and examined by PCR. The results showed that the ALV-A/B antibody was positive for 13.24% at the age of 240 days and REV antibody was positive at the age of 120 days and increased with age,while ALV-J antibody was always negative. Positive rate / of antigen p27 was from 34.21% to 66.18%. The mortality was 32% from egg laying stage to the age of 240 day. Numerous similar nodular lesions and resembling tumors in one or more tissues were seen at necropsy and some sick chickens＇ sciatic nerve was edematous. Histopathology findings revealed large islands of lymphocytes in tissues con- taining tumors. Among 16 samples, 14 samples were detected positive for AI.V-J （87.50%）, 13 samples for MDV （81.25%） ,2 samples for REV （12.5%） and co-infection rate was 6.30%. All the findings observed in this case were considered to be related with the MDV,REV and ALV mixed infection.

关 键 词：芦花鸡 肿瘤病 ELISA 病理组织学 PCR检测 

分 类 号：S858.31[农业科学—临床兽医学]