抑制性削减技术研究CD3/CD28单抗共刺激人T淋巴细胞基因差异表达  

Study on differentially expressed genes in human T cells coactivated by anti-CD3 and anti-CD28 antibodies using SSH technique

作　　者：李新平[1] 陈曦[1] 王丽娜[1] 李创宏[1] 张文龙[1] 张淼涛[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨陵712100

出　　处：《中国兽医学报》2011年第11期1609-1613,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30471250)

摘　　要：为了研究参与人T细胞活化、增殖、信号转导的相关基因,以正常人初始T细胞作为对照样本,以抗人CD3抗体联合抗人CD28抗体激发的人T细胞作为试验样本,进行抑制性削减杂交。结果筛选到43个侯选克隆,对其单向测序后,经过与GenBank同源序列比较,证实了6个克隆参与了T细胞活化增殖和信号转导的下游通路,分别为CCND2、Rho A、IRF4、FKSG44、EBP、PRPPS2;13个克隆所可能编码的基因与T细胞活化、分裂增殖、分化有关,另外24个克隆在GenBank无法查到对应的同源基因,可能代表了新基因。对这些基因的进一步研究,有助于阐释T细胞活化、增殖的信号转导机制,为了解体内活化T细胞参与的生理和病理过程,具有一定的指导意义。To study genes involved in human T ceil proliferation,differentiation and signal transduction, a suppression subtractive hybridization （SSH） technique was developed to screen the specific （differentially expressed） transcrips of T cells coactivated with anti-CD3 and anti-CD28 antibodies serving as ＂tester＂,and the normal T cells as ＂driver＂ in the experiments. This experiment revealed a total of 43 gene regulations. Only 6 （13.9 % ） of these regulated genes had OMIM records annotated as T cell activation genes （CCND2, Rho A, IRF4, FKSG44, EBP, PRPPS2）. The possi- Ble representing genes of 13 clones were not known T cell activation genes previously undocumented roles in T cell activation. These ESTs with so many T cell activation-associated genes suggest that some of the protein products.of these EST transcripts may also function during T cell activation. The majority of these ESTs （19/43） correspond to transcripts whose complete coding sequences have been determined recently or at least partially characterized （＂known transcripts＂）. Twenty four ESTs were not linked to newly published cDNAs by continuous updating of GenBank during this study. They could represent the new genes. In theory, such approaches have shown a promise for the systematic annotation of unknown ORFs from T cell activation genes and should be possible to improve our ability to assign unknown genes to biological processes by systematically examining gene regulation under multiple conditions and quantitatively determining optimal experimental. Further studies on the functions of these genes can help to understand the mechanisms of T cell proliferation and differentiation,and the physiological and pathological course concerned with activated T cells.

关 键 词：抑制性削减杂 T细胞活化 信号转导 CD3抗体 CD28抗体 

分 类 号：S852.4[农业科学—基础兽医学]