1α,25-(OH)_2D_3影响体外培养OB增殖分化的钙离子通道机制  

Calcium channel mechanism involved in proliferation and differentiation of osteoblasts affected by 1α,25-(OH)_2D_3 in vitro

作　　者：顾建红[1] 蒋杉杉[1] 王世涛[1] 闫汶[1] 王怡[1] 刘宗平[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2011年第11期1627-1630,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972229;30571364);江苏省自然科学基金资助项目(BK2008214;BK2009736)

摘　　要：为研究1α,25-(OH)2D3影响体外培养成骨细胞(Osteoblasts,OB)增殖分化是否存在L-型钙离子通道机制。在体外培养SD大鼠OB基础上,添加不同浓度的1α,25-(OH)2D3(0、10-11、10-9、10-7 mol/L)或和10-8 mol/L硝苯地平(NIF)作用24、48、72h。采用MTT法测定OB增殖率,PNPP法测定碱性磷酸酶(ALP)活性。结果显示,1α,25-(OH)2D3剂量依赖性地抑制OB增殖、促进ALP活性;10-8 mol/L NIF单独作用亦能抑制OB增殖、促进ALP活性;并且在培养早期(24、48h)能消除10-9 mol/L 1α,25-(OH)2D3对OB增殖的抑制效应及对ALP活性的促进效应。结果表明,1α,25-(OH)2D3能够抑制OB增殖、促进其分化;并且此过程涉及L-型钙离子通道机制。To study whether L-type calcium channel mechanism is involved in proliferation and differentiation of oste- oblasts fOB） affected by 1α,, 25-（OH）2D3 in vitro, different concentrations of 1α,, 25-dihydroxyvitamin D3 or and nifedipine （NIF） were added into the culture after 80% cell fusion. 24,48 and 72 h after cultured, the proliferation and the alkaline phosphatase （ALP） activity of OB were evaluated. The results showed that,1α,, 25-（OH）2 D3 inhibi- ted the proliferation and induced ALP activity dose dependently. Similarly, 10 8 mol/L NIF inhibited OB proliferation and induced ALP activity alone. At the same time,10-8 mol/L NIF eliminated the inhibitory effect on OB prolifera- tion and the promotion effect on ALP activity induced by 1α,, 25- （ OH ）2 D3. It was concluded that, 1α, 25- （ OH ） 2 D3 could inhibit proliferation and induce differentiation of OB,and this process involves l.-type calcium channel mechanism.

关 键 词：成骨细胞 1α 25-(OH)2D3 碱性磷酸酶 硝苯地平 钙离子通道 

分 类 号：S852.2[农业科学—基础兽医学]