高钙日粮对青年蛋鸡肾脏中XOD基因mRNA表达的影响  

Effect of high calcium diet on expression of XOD mRNA in renal of growers

作　　者：郭小权[1] 曹华斌[1] 黄克和[2] 胡国良[1] 雷鹏[1] 田山[1] 国鑫[1] 

机构地区：[1]江西农业大学动物科学技术学院,江西南昌330045 [2]南京农业大学畜禽营养代谢病研究室,江苏南京210095

出　　处：《中国兽医学报》2011年第11期1663-1666,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30860212);江西省自然基金资助项目(2007GQN0235);江西农业大学自然基金(赣农大科发[2007]10号)

摘　　要：选择35日龄伊沙蛋鸡100羽,随机均分为对照组(含钙1.00%)和高钙组(含钙3.78%)。于试验30d每组选取体质量相近的试验蛋鸡5羽,取肾脏样品,应用半定量逆转录聚合酶链式反应技术测定XOD mRNA在肾脏中的表达水平。结果显示:高钙组肾脏中XOD mRNA对β-actin mRNA的相对丰度显著高于对照组。说明高钙日粮可促进鸡肾脏的XOD mRNA的表达。One hundred healthy growers of 35-days-old were randomly divided into two groups, which were fed with normal calcium diet （control, 1.00% calcium）, high calcium diet （HC, 3.78 % calcium）, respectively. The XOD mRNA expression level in renal of growers were assayed at 30 d post-treatment by semi-quantification and reverse transcription polymerase chain reaction （RT-PCR）. The results showed the XOD mRNA in renal of HC group significantly increased compared with the control. The results indicated that high dietary calcium could stimulate the expressing of XOD mRNA in renal.

关 键 词：高钙日粮 XOD 肾脏 基因表达 

分 类 号：S856.5[农业科学—临床兽医学]