屋尘螨对支气管上皮细胞通透性的影响  

Effect of house dust mites on permeability in human bronchial epithelial cells in vitro

作　　者：高元妹[1] 夏旸[1] 沈湘波[1] 张平[2] 赵海金[1] 蔡绍曦[1] 

机构地区：[1]南方医科大学南方医院呼吸科,广州510515 [2]东莞市人民医院呼吸科,523018

出　　处：《国际呼吸杂志》2011年第20期1526-1529,共4页International Journal of Respiration

基　　金：本课题受广东省医学科研基金资助（A2008779）;东莞市医疗卫生重点课题基金资助（200910515021）;国家自然科学基金资助（30971328）

摘　　要：目的探讨屋尘螨对支气管上皮细胞通透性的影响。方法通过EI，ISA、荧光定量PCR检测屋尘螨刺激人的支气管上皮细胞后对血管内皮生长因子（VEGF）表达的影响，通过检测细胞的跨膜电阻了解屋尘螨对人的支气管上皮细胞通透性的影响。结果屋尘螨可以上调人的支气管上皮细胞VEGF的表达，且呈明显的时间依赖性和浓度依赖性，并与支气管上皮细胞的通透性呈正相关，VEGF的单克隆抗体可以明显阻断这一效应。结论屋尘螨通过上调VEGF的表达增加了对支气管上皮细胞的通透性。Objective To investigate whether house dust mites （HDM） increase pemeability in bronchial epithelial permeability. Methods We estimated HDM induced VEGF release in NHBE by using an ELISA and VEGF mRNA expression by using an RT PCR reaction. The influence of HDM on NHBE barrier function was estimated by measuring electrical resistance with an electric cell substrate impedance sensing system. Results Our results demonstrate that HDM induces VEGF release in NHBE in a timedependent and concentration-dependent manner. HDM decreased electrical resistance across NHBE morlolayers. Neutralizing antibodies to VEGF significantly inhibited the decrease in NHBE electrical resistance caused by HDM. Conclusions HDM increases permeability of bronchial epithelial monolayer via VEGF induction.

关 键 词：屋尘螨 血管内皮生长因子 支气管上皮细胞通透性 

分 类 号：R562.25[医药卫生—呼吸系统]