不同频率间歇低氧下大鼠肝脏氧化应激损伤及Tempol的干预作用  

A study of rat liver oxidative stress injury with different frequencies intermittent hypoxia and the effect of Tempoi

作　　者：姜秋芳[1] 陈宝元[1] 董丽霞[1] 林慧[1] 孔铖英[1] 张静[1] 郑立文[1] 

机构地区：[1]天津医科大学总医院呼吸科,300052

出　　处：《国际呼吸杂志》2011年第20期1546-1549,共4页International Journal of Respiration

基　　金：国家自然科学基金资助项目（50770954）

摘　　要：目的探讨不同频率间歇低氧（IH）对大鼠肝脏氧化应激损伤差异和Tempol的干预作用及可能机制。方法应用慢性间歇低氧（CIH）大鼠模型，模拟OSAS周期性间歇低氧／再氧和病理生理过程。56只雄性Wistar大鼠随机分为不同频率IH组（IH1，IH2，IH3，IH4，频率依次为10、20、30、40次／h），30T组（30次／h低氧Tempol干预组），30N组（30次／h低氧生理盐水对照组），以及常氧对照组（NC组），共7组，每组8只，IH各组最低氧浓度均为5％。暴露6周后，测定大鼠肝脏丙二醛（MDA）、超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）的水平。结果①4个不同频率IH组MDA水平均高于NC组（P值均〈0．05），SOD、TAOC活性均低于NC组（P值均〈0．05），且随着IH频率的升高，MDA水平逐渐升高，SOD、T—AOC活性逐渐下降，除IH3组和IH4组MDA、SOD、T—AOC水平差异均无统计学意义外（P值均〉0．05），其余IH组两两比较，以上指标差异均有统计学意义（P值均〈0．05）。②与IH3组、30N组比较，30T组MDA水平下降（P〈0．05），SOD、T—AOC活性升高（P值均〈0．05），且与NC组比较差异无统计学意义（P值均〉0．05）。③不同频率IH组MDA水平与SOD、T-AOC活性均呈负相关（r分别为-0．721，-0．653，P值均=0．000）。结论不同频率CIH对大鼠肝脏氧化应激损伤的程度不同，但并不是频率越高，损伤越重；Tempol能预防CIH大鼠肝脏氧化应激损伤的发生。Objective To study the effect of different frequencies intermittent hypoxia on malondialdehyde （MDA）, superoxide dismutase （SOD） and total antioxidant capacity （T-AOC）of rat liver and to investigate the effect of Tempol. Methods To establish a chronic intermittent hypoxia （CIH） animal model in rats,in order to mimic the intermittent hypoxia/reoxygenation （IH/ROX） of obstructive sleep apnea syndrome in humans. 56 healthy male Wistar rats were randomly assigned to seven groups：4 different IH frequencies groups （IH1：10 times/h, IH2：20 times/h, IH3：30 times/h,IH4：40 times/h with 5% O2）,the IH3＋Tempol group （30T group）,the IH3＋normal saline control group （30N group） and normoxia control group （NC group） ,each group of eight rats. After the experiment,we measured the levels of MDA,SOD and T AOC in rat liver homogenate. Results The level of MDA in 4 different IH frequencies groups was higher than in NC group （all P 〈0.05） ,the activity of SOD,T AOC were lower than in NC group （all P d0.05）. And there was a trend as the IH frequency increased,the level of MDA was gradually increased, the activity 9f SOI3 and T-AOC were gradually decreased. The differences of MDA, SOD,T-AOC level were statistical significant between each two different IH frequencies groups （all P 〈0.05） ,except the differences between IH3 group and IH4 group （all P 〉0.05）. Compared with IH3 group,30N group,the level of MDA in 30T group was decreased （ P -（0.05） ,the activity of SOD,T-AOC were increased （all P 〈0.05）,and the levels of MDA, SOD,T-AOC in 30T group were not significant with in NC group （all P 〉0.05）. The level of MDA in 4 different IH frequencies groups was negative correlation with the activity of SOD, T-AOC （ r were -0. 721, -0. 653 respectively, all P = 0. 000）. Conclusions Different CIH frequencies had different degrees of injury of oxidative stress on rat liver,but the relationship between the degree of injury on liver and the CIH frequencies wa

关 键 词：间歇低氧 氧化应激 阻塞性睡眠呼吸暂停综合征 TEMPOL 

分 类 号：R766[医药卫生—耳鼻咽喉科]