用Walker 256大鼠乳腺癌细胞建立大鼠胫骨癌痛模型  

Establishing Rat Model of Tibial Cancer Pain with Walker 256 Mammary Gland Carcinoma Cells

作　　者：陈雯[1] 王静捷[1] 黄宇光[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院麻醉科,北京100730

出　　处：《协和医学杂志》2011年第4期350-356,共7页Medical Journal of Peking Union Medical College Hospital

基　　金：中央保健专项基金(B2009B076)

摘　　要：目的探讨用Walker 256大鼠乳腺癌细胞建立大鼠胫骨癌痛模型的可行性。方法将36只SD雌性大鼠,随机分为肿瘤组(n=8)、假手术1(n=10)、假手术2(n=10)和正常对照组(n=8)。将含1×105 Walker 256大鼠乳腺癌细胞悬液注入大鼠胫骨上段制备骨癌痛模型,观察疼痛行为学[包括机械缩足反射阈值(mechanical paw with-drawal threshold,MWT)和热缩足反应潜伏期(paw withdrawal thermal latency,PWTL)]、骨放射学和组织学变化。结果疼痛行为学、影像学、组织学观察结果证实,Walker 256大鼠乳腺癌细胞能成功制备大鼠胫骨癌痛模型。肿瘤组造模后第7天开始MWT显著降低,为(37.59±2.02)g,与假手术组1、2和正常对照组比较,P<0.01;第11天PWTL显著降低,为(6.87±1.00)s,与假手术组1、2和正常对照组比较,P<0.01。结论用Walker 256大鼠乳腺癌细胞能够成功建立大鼠胫骨癌痛模型。Objective To explore the feasibility of establishing a rat model of tibial cancer pain with Walker 256 mammary gland carcinoma cells.Methods Totally 36 female SD rats were randomly divided into 4 groups：Sarcoma group（n=8）,Sham 1 group（n=10）;Sham 2 group（n=10）;and Nave group（n=8）.In each treatment group,105 syngenenic Walker 256 mammary gland carcinoma cells,ascetic fluid without tumor cells,and normal saline were respectively injected into the tibia medullary cavity via intercondylar eminence.The pain behaviors including mechanical paw withdrawal threshold（MWT） and paw withdrawal thermal latency（PWTL）,bone radiology,and bone histology were evaluated.Results Observational results via pain behaviors,radiology,and histology demonstrated that rat model of tibial cancer pain was successfully established with Walker 256 mammary gland carcinoma cells.MWT significantly decreased to 37.59±2.02g on the 7th day after tumor cell injection in the Sarcoma group（P0.01 compared with Sham 1 group,Sham 2 group,and Nave group）.PWTL significantly decreased to 6.87±1.00s on the 11th day（P0.01 compared with Sham 1 group,Sham 2 group,and Nave group）.Conclusion Rat model of tibial cancer pain can be successfully established with Walker 256 mammary gland carcinoma cells.

关 键 词：WALKER 256肿瘤细胞 骨癌痛模型 

分 类 号：R441.1[医药卫生—诊断学]