姜黄素对大鼠慢性酒精性胰腺炎的作用和机制研究  

Role and mechanism of curcumin on rats with chronic pancreatitis

作　　者：彭晓华[1] 周旭春[1] 潘慧[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016

出　　处：《中华胰腺病杂志》2011年第5期343-345,共3页Chinese Journal of Pancreatology

基　　金：重庆市卫生局科研项目（062096）;重庆医科大学校办课题（QD200508）

摘　　要：目的观察姜黄素对大鼠实验性慢性胰腺炎的干预作用。方法18只1月龄雄性SD大鼠按数字表法随机分为对照组、ANP组和姜黄素组，每组6只。以25％乙醇代水饮12周后每周1次腹腔注射脂多糖（2mg／kg体重）、共4次的方法建立慢性胰腺炎模型。姜黄素组制模同时饲以姜黄素。17周后处死动物。测血糖浓度，常规胰腺病理检查及胶原纤维染色，检测胰腺组织淀粉酶和脂肪酶水平。采用RT—PCR法检测胰腺组织转化生长因子β1（TGF-β1）mRNA表达。结果对照组、ANP组和姜黄素组胰腺病理评分分别为1．17±0．41、7．33±2．58和3．50±1．05，姜黄素组较ANP组降低（P〈0．01），但仍高于对照组（P〈0．05）。各组血糖值无统计学差异。姜黄素组胰腺组织匀浆淀粉酶和脂肪酶含量分别为（7348±102）、（14135±1272）U／g，均高于ANP组的（5428±547）和（9123±1250）U／g（P值均〈0．05）。对照组、ANP组和姜黄素组胰腺TGF-β1mRNA表达量分别为0．618±0．019、0．818±0．012、0．745±0．088，ANP组显著高于对照组和姜黄素组（P值均〈0．01）。结论在长期摄入乙醇的基础上反复腹腔注射脂多糖可诱导慢性胰腺炎；姜黄素可能通过抑制TGF-β1表达而产生抗胰腺纤维化的作用。Objective To investigate the effect of curcumin on chronic pancreatitis in rat. Methods Eighteen one-month old male SD rats were randomly divided into control group, ANP group and curcumin group with 6 rats in each group. After fed by 25% alcohol for twelve weeks, the rats in ANP group were intraperitoneally injected with lipopolysaccharides （ LPS, 2 mg/kg body weight） once a week for 4 weeks. The rats in eurcumin group were fed with curcumin when they were intraperitoneally injected with lipopolysaccharides. Seventeen weeks later, the rats were sacrificed. Serum glucose was measured, pancreatic morphology was routinely examined, collagen deposition was determined by Masson stain, and the amylase and lipase in pancreatic tissue were evaluated, TGF-131 mRNA was determined by using RT-PCR. Results The pathological score of control group, ANP group and eurcumin group was 1.17 ±0.41,7.33 ±2.58 and 3.50 ± 1.05, and the score in eurcumin group was lower than that of ANP group （ P 〈 0.01 ）, but which was higher than that in control group （ P 〈 0.05 ）. There was no difference in the serum glucose among the groups. The levels of amylase and lipase in pancreatic tissue were （ 7348± 102 ）, （ 14135 ± 1272 ） U/g, which were significantly higher than those in ANP group [ （ 5428 ± 547 ） and （ 9123 ± 1250 ） U/g, P 〈 0.05 ]. The expressions of TGF-β1 mRNA in control group, ANP group and curcumin group were 0.618 ± 0.019, 0.818 ±0. 012, 0. 745 ± 0. 058, and the values in ANP group were significantly higher than those in control group and curcumin group （P 〈 0.01 ）. Conclusions On the basis of long term ethanol intake, repeatedly intraperitoneal injection of LPS can induce chronic pancreatitis, and curcumin has anti-fibrosis effect possibly by its inhibitory effect on the expression of TGF-β1.

关 键 词：姜黄素 慢性胰腺炎 内毒素类 乙醇 

分 类 号：R285.5[医药卫生—中药学]