PLGA/PCL/nHA生物支架复合兔BMSCs体外培养的实验研究  

Biocompatibility and Osteogenesis of Biomimetic PLGA/PCL/nHA Composite Scaffolds for Bone Tissue Engineering in vitro

作　　者：乔广艳[1,2] 沈庆平 苏俭生[2] 徐红珍[2] 孙俊[2] 

机构地区：[1]上海市口腔病防治院修复科,上海200001 [2]同济大学口腔医学院修复科,上海200072

出　　处：《口腔颌面外科杂志》2011年第5期326-329,333,共5页Journal of Oral and Maxillofacial Surgery

基　　金：上海市科学技术委员会资助项目(0852nm03600)

摘　　要：目的:研究新型复合支架聚乳酸-聚乙醇酸/聚己内酯/纳米羟基磷灰石(PLGA/PCL/nHA)的生物相容性,探讨其作为细胞培养材料和骨组织工程支架的可行性。方法:将兔骨髓基质细胞(bone marrow stromal cells,BMSCs)接种于PLGA/PCL/nHA复合支架上,体外共同培养后,MTT法检测BMSCs增殖活性,电镜下观察PLGA/PCL/nHA复合支架材料表面和孔隙内细胞附着情况。结果:BMSCs在PLGA/PCL/nHA复合支架材料上生长良好。PLGA/PCL/nHA材料表面和孔隙内均有细胞附着,细胞已伸展成梭形或多角形,伸出伪足黏附于材料上。结论:新型PLGA/PCL/nHA复合支架材料生物相容性好,是一种良好的组织工程支架材料,可作为种子细胞的载体应用于骨组织工程中。Objective： Myriad scaffolding materials and compositions have been and are still being tested for the regeneration of each very specific hard or soft tissue.The objective of this study was to examine a novel composite poly（lactic-co-glycolic acid） / poly（e—caprolactone） / nano-carbonate hydroxyapatite（PLGA/PCL/nHA） on the initial behavior of bone marrow stromal cells（BMSCs） and evaluate the proliferation and differentiation of BMSCs grown on composite scaffold in vitro.Methods： BMSCs cells were cultured on PLGA/PCL/nHA in vitro,cell viability was assessed by MTT reduction assay.Cell adhesion on PLGA/PCL/nHA was observed by scanning electron microscopy（SEM）.Results： BMSCs cells viability were not affected,the proliferation and growth were not affected by PLGA/PCL/nHA,and BMSCs cells could be attached to with a spindle shape or a polygonal shape and extended the pseudopod on PLGA/PCL/nHA.Conclusions： The composite scaffolds is biocompatible and crucial in regulating cellular functions,including survival,adhesion,proliferation,and the like.

关 键 词：聚乳酸-聚乙醇酸/聚己内酯/羟基磷灰石复合支架 骨髓基质细胞 生物相容性 MTT 

分 类 号：R318.08[医药卫生—生物医学工程]