鸡贫血病毒vp3基因的克隆和分子特征  

Cloning and molecular characteristics of chicken anemia virus vp3 gene

作　　者：吴海波[1] 沃恩康[1] 尤金彪[1] 王怡婷[1] 王巧刚[1] 吴南屏[2] 郭潮潭[1] 

机构地区：[1]浙江省医学科学院生物工程研究所,杭州310013 [2]浙江大学医学院附属第一医院传染病诊治国家重点实验室, 杭州310003

出　　处：《国际流行病学传染病学杂志》2011年第5期294-298,共5页International Journal of Epidemiology and Infectious Disease

基　　金：国家自然科学基金（81071849）;卫生部科研基金（WK32009-2-016）

摘　　要：目的获得来源于浙江地区鸡组织中的鸡贫血病毒（CAV）筇基因并分析其变异情况。方法根据GenBank登录的vp3序列设计特异引物，利用PCR方法从鸡组织中获得7个CAV的硪，基因克隆，将其克隆到载体进行测序。结果40份鸡血清中共检出CAV阳性16份，阳性率40．0％。序列测定结果表明，叼妇基因长366bp，编码121个氨基酸，与GenBank登录的其他毒株目妒基因的核苷酸序列同源性为97．O％。100．0％，氨基酸序列同源性为95．0％-100．0％。结论通过PCR方法扩增获得了CAVvp3基因，w3基因及VP3蛋白总体上非常保守。Objective To obtain the chicken anemia vius（CAV） vp3 gene from Zhejiang region chicken and analyze its variation. Methods The specific primers were designed according to the published sequences in GenBank. By PCR, 7 clones of CAV vp3 gene were obtained from CAV-infected chicken. Results 16 CAV-positive chicken serum were detected in 40 samples, and the positive rate was 40.0%. Sequencing results showed that vp3 gene consisted of 366 bp, coded 121 amino acids, and shared 97.0%-100.0% nucleotide homology and 95.0%-100.0% protein homolo- gy with that of the other strains submitted to GenBank. Conctusions CAV vp3 gene is obtained by PCR, vp3 gene and VP3 protein are very conservative.

关 键 词：鸡贫血病毒 VP3基因 克隆 分子特征 

分 类 号：S852[农业科学—基础兽医学]