乙型肝炎病毒前S1抗原与其血清标志物及DNA的关系分析  

Relations between HBV Pre-S1 antigen and its serological markers and HBV DNA

作　　者：刘波[1] 王君[1] 邱小华[1] 

机构地区：[1]汕头大学医学院第二附属医院检验科,515041

出　　处：《国际流行病学传染病学杂志》2011年第5期308-310,共3页International Journal of Epidemiology and Infectious Disease

摘　　要：目的探讨HBV前S1抗原（Pre-S1）与HBV血清标志物（HBV-M）及HBVDNA的关系。方法采用ELISA法检测880例乙型肝炎患者血清Pre-S1和HBV-M，采用荧光定量PCR法检测HBV DNA载量，并分析三者间的相关性。结果在880例乙型肝炎患者中，前S1抗原和HBeAg的阳性率分别为56．25％和37．27％，HBVDNA总检出率为63．52％。在HBsAg、HBeAg和HBcAb三者均阳性的患者中，Pre-S1和HBVDNA阳性率分别为91．14％和94．62％，HBeAg与Pre-S1和HBVDNA阳性诊断率差异无统计学意义（X^2=1．69，P〉0．05）；在HBsAg和HBeAg两项阳性的患者中，上述三种指标阳性检出率差异也无统计学意义（X^2=2．13，P〉0．05）；而在HBeAg阴性的患者中，Pre-S1和HBVDNA阳性诊断率明显较低。在495例Pre-S1阳性患者中检出HBVDNA阳性456例（92．12％），阳性检出率与Pre-S1匹配度较高（X^2=2．54，P〉0．05）。Pre-S1检出率与HBVDNA载量呈明显正相关（r=0．84，P〈0．01）。结论HBeAg阳性患者，Pre-S1和HBVDNA阳性检出率较高。Pre-S1与HBVDNA阳性诊断率的一致性较好，能够较好地反映HBV在体内的复制状态，在乙型肝炎的诊断和治疗中可能具有重要临床意义。Objective To discuss the relations among Pre-S1 antigen, HBV serological markers and HBV DNA. Methods Pre-S1 antigen and serological markers of 880 hepatitis B patients were detected by ELISA. HBV DNA load was detected by fluorescent quantitative PCR（FQ-PCR）. The relations were analyzed. Results In 880 hepatitis B pa- tients, the positive rate of Pre-S1 antigen and HBeAg was 56.25% and 37.27% respectively.The detection rate of HBV DNA was 63.52%. In the patients with HBsAg（ ＋ ）, HBeAg（ ＋ ）, and anti-HBe（ ＋ ） , the positive rate of Pre-S1 and HBV DNA were 91.14% and 94.62% respectively. There were no statistical differences in the detection rates among HBeAg, Pre-S1 and HBV DNA （X^2 = 1.69,P 〉 0.05）. In the patients with HBsAg（ ＋ ）, HBeAg（ ＋ ）, there were no statistical differences in the detection rates among the above three indexes （X^2 = 2.13, P 〉 0.05）. In the patients with HBeAg negative, the detection rate of Pre-S1 and HBV DNA were obviously lower. In the 495 Pre-S1 positive patients, there were 456 patients with HBV DNA positive（92.12% ）. The positive rate of HBV DNA and Pre-Sl matched well （ X^2 = 2.54, P 〉 0.05）. The positive correlation between Pre-S1 detection rate and HBV DNA viral load was observed（ r = 0. 84, P 〈 0.01 ）. Conclusions In the HBeAg positive patients, the positive detection rate of Pre-S1 and HBV DNA is high. The consistency of the positive diagnostic rate of Pre-S1 and HBV DNA is good. Pre-S1 can reflect the HBV repli- cation in HBeAg positive patients, and may have great clinical significance in the diagnosis and treatment of hepatitis.

关 键 词：肝炎病毒 乙型 前S1蛋白 血清标记物 HBV DNA 

分 类 号：R51[医药卫生—内科学]