红锥、白椎、大叶栎3个树种分子鉴定及亲缘关系的ISSR分析  

Molecular Identification of Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii Using ISSR-PCR Method

作　　者：刘海龙[1,2,3] 陈晓明[1,2,3] 覃子海[1,2,3] 杨开太[1,2,3] 林建新[1,2,3] 黄金使[1,2,3] 

机构地区：[1]广西壮族自治区林业科学研究院,广西南宁530002 [2]国家林业局中南速生材繁育实验室,广西南宁530002 [3]广西优良用材林资源培育重点实验室,广西南宁530002

出　　处：《安徽农业科学》2011年第30期18419-18420,18450,共3页Journal of Anhui Agricultural Sciences

基　　金：广西林科院基本业务费项目"红锥群体遗传学研究"(林科200901)

摘　　要：[目的]进行红锥、白椎、大叶栎3个树种的分子鉴定研究。[方法]利用ISSR-PCR方法构建红锥(Castanopsis hystrix)、白椎(Cas-tanopsis carlesii Hayata)、大叶栎(Quercus griffithii Hook)3个树种的DNA指纹图谱,根据各个体的Nei氏遗传距离矩阵,利用UPGMA法进行聚类分析。[结果]从50个ISSR引物中筛选出6个多态性引物用于ISSR-PCR扩增,共扩增出86条不同DNA带,平均每个引物扩增的DNA带的数目为10.75条,多态性条带数目为53条,占总条带数的61.2%。其中,有5个引物可扩增出差异条带和特异条带,能准确地鉴定上述3个树种。应用DPS软件计算供试材料的遗传距离界于0.166 67～0.809 52之间,平均为0.563 57。[结论]ISSR-PCR方法可以解决红锥、白锥、大叶栎的鉴定问题。[Objective] This research aimed to develop molecular identification method for Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii.[Method] DNA fingerprints of C.hystrix,C.carlesii and Q.griffithii were established using ISSR-PCR method.Cluster Analysis was carried out using UPGMA method based on Nei＇s genetic distances among each individual.[Result] Six polymorphic primers were selected from 50 ISSR primers for ISSR-PCR amplification,and totally 86 discernible DNA bands were amplified with 53 polymorphic bands,accounting for 61.2% of the total.The average number of DNA bands amplified by each primer was 10.75.Specifically,totally 5 primers had amplified differential bands and specific bands,which were able to accurately identify C.hystrix,C.carlesii and Q.griffithii.As calculated by DPS v3.01 software,the genetic distances among test materials were ranged from 0.166 67 to 0.809 52,with an average of 0.563 57.[Conclusion] ISSR-PCR method can be used to identify C.hystrix,C.carlesii and Q.griffithii effectively.

关 键 词：壳斗科 红锥 白椎 大叶栎 ISSR 指纹图谱 

分 类 号：S718.46[农业科学—林学]