黄鳝载脂蛋白apoAI基因的克隆及序列分析  

Molecular Cloning and Sequence Analysis of An apoAI Gene in Rice Field Eel Monopterus albus

作　　者：邱庆崇[1] 耿小雪[1] 刘成都[1] 孙文秀[1] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025

出　　处：《湖北农业科学》2011年第21期4500-4502,共3页Hubei Agricultural Sciences

基　　金：湖北省教育厅优秀中青年基金项目(Q200712005)

摘　　要：以黄鳝(Monopterus albus)肝脏为研究材料,提取总RNA,利用同源克隆技术获得了黄鳝的载脂蛋白apoAI基因的部分编码区(GenBank登录号:HQ603782)和3′UTR,其cDNA序列长度为1 145 bp,其中编码区长度为772 bp,编码256个氨基酸。序列同源性分析表明,黄鳝的apoAI基因的氨基酸序列与其他鱼类的apoAI基因的氨基酸序列具有非常高的同源性,是载脂蛋白家族的一个新成员。黄鳝apoAI基因的克隆为进一步深入研究该基因的功能积累了基础资料,在黄鳝育种及医药研制方面具有潜在的应用价值。Part of coding and 3′ UTR sequences of apoAI gene（GenBank accession： HQ603782） was cloned from the total liver RNA of rice field eel（Monopterus albus）.Sequence analysis showed that the length of this cDNA sequences was 1 145 bp,including 772 bp coding region and encoded 256 amino acids.Homology analysis showed that there was high sequences similarity between the Monopterus albus and other fishes’ apoAI genes,which were belong to the apolipoprotein family.Cloning of the Monopterus albus’ apoAI gene gave the basis for further inverstigation its function,and had the potential application in Monopterus albus’ breeding and medical usage

关 键 词：黄鳝(Monopterus albus) 载脂蛋白 APOAI 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学] Q959.482