提取方法与部位对浙贝母基因组DNA提取质量的影响  

Effects of Extraction Method and Tissues on the Quality of Genomic DNA in Fritillaria thunbergii

作　　者：周洁[1] 王晓飞[2] 金晓霞[2] 王忠华[2] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018 [2]浙江万里学院生物技术研究所,浙江宁波315100

出　　处：《湖北农业科学》2011年第21期4503-4505,共3页Hubei Agricultural Sciences

基　　金：浙江省科技厅重点项目(2009C12088)

摘　　要：探讨了改良CTAB法、SDS法、高盐低pH法和试剂盒法对浙贝母(Fritillaria thunbergii Miq.)基因组DNA的提取效果。综合比较了各方法提取的DNA浓度、纯度、完整性,结果表明高盐低pH法是比较适合浙贝母新鲜叶片基因组DNA提取的方法。比较了分别以叶片和鳞茎为材料提取的DNA质量,发现以新鲜叶片为材料提取的DNA浓度和纯度较高,更适合用于提取浙贝母基因组DNA。Modified CTAB method,high salt low pH method,SDS method and DNA Mini-prep kit method were used to extract genomic DNA from Fritillaria thunbergii Miq.Comprehensive comparison of the concentration,purity and integrity of genomic DNA extracted by various methods showed that high salt low pH method was the suitable method for extracting DNA from F.thunbergii.Comparison between the DNA extracted from fresh leaves and bulb revealed that DNA obtained from fresh leaves was with higher concentration and purity,thus fresh leaves were better materials for DNA extraction of F.thunbergii

关 键 词：浙贝母(Fritillaria thunbergii Miq.) 基因组DNA 提取 

分 类 号：S567.231[农业科学—中草药栽培]