HPA、bFGF及VEGF诱导血管生成在鼻咽血管纤维瘤扩展性生长中的作用  

Significance of heparanase,bFGF and VEGF in angiogenesis for nasopharyngeal angiofibroma outspreding

作　　者：王静[1] 易自翔[1] 赵德安[2] 陈缪安[3] 李志春[1] 

机构地区：[1]福建医科大学附属第一医院耳鼻咽喉-头颈外科,福州350005 [2]厦门市第一医院耳鼻咽喉科 [3]福建医科大学附属闽东医院耳鼻咽喉-头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2011年第21期970-973,976,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：福建省财政厅科研基金(No:2009B011)

摘　　要：目的:探讨鼻咽血管纤维瘤(JNA)中乙酰肝素酶(HPA)、碱性成纤维细胞生长因子(bFGF)、血管内皮细胞生长因子(VEGF)的表达及其诱导血管生成在肿瘤扩展性生长中的作用。方法:应用免疫组织化学法检测30例JNA组织和20例下鼻甲组织中HPA、bFGF、VEGF和CD105的表达,以CD105的染色计算新生血管的微血管密度(MVD),结合临床分期进行分析。结果:JNA组织中HPA、bFGF及VEGF的阳性表达率高于下鼻甲组织(P<0.05);Ⅲ～Ⅳ期JNA组织HPA、bFGF及VEGF的阳性表达率明显高于Ⅰ～Ⅱ期(P<0.05);bFGF和VEGF的表达均与HPA呈正相关(r=0.499、0.582,均P<0.05);JNA组织中HPA、bFGF共阳性表达组的MVD值显著高于单一阳性及共阴性表达组(P<0.05);HPA、VEGF共阳性表达组的MVD值亦高于单一阳性及共阴性表达组(P<0.05)。结论:HPA可通过释放bFGF及VEGF来诱导血管生成以促进肿瘤扩展性生长,针对其进行靶向治疗,抑制肿瘤血管生成,将是JNA辅助治疗的一个新方向。Objective：To observe the expression of HPA,bFGF and VEGF in nasopharyngeal angiofibroma,and then explore its significance of inducing angiogenesis in the tumor＇s expansibility growth.Method： The expression of heparanase,bFGF,VEGF and CD105 were examined in 30（Ⅰ-Ⅱperiod 9 cases,Ⅲ-Ⅳperiod 21 cases）samples from nasopharyngeal angiofibroma and 20 inferior turbinate tissues by immunohistochemical staining technique.The microvasculars density（MVD） were measured by the immunohistostaining of CD105.The MVD was analyzed with the clinical stage.Result：The positive rates of the HPA,bFGF and VEGF expression in JNA tissues were significantly higher than that in inferior turbinate group（P0.05）.The positive rates of HPA,bFGF and VEGF expression in Ⅲ-Ⅳ period were obviously higher than that in Ⅰ-Ⅱ period（P0.05）.The expression of bFGF and VEGF in JNA tissues was respectively positive correlated with the HPA（r=by 0.499,0.582,P0.05）;In JNA tissues,the mean MVD in both HPA and bFGF positive group was higher than each one single positive group or both negative express group（P0.05）.And the mean MVD in both HPA and VEGF positive group was higher than each one single positive group or both negative express group（P0.05）.Conclusion：HPA can induce angiogenesis to promote tumor growth by releasing bFGF and VEGF.Targeting the HPA can be a new direction in JNA adjuvant treatment.

关 键 词：鼻咽血管纤维瘤 乙酰肝素酶 碱性成纤维细胞生长因子 血管内皮细胞生长因子 血管生成 

分 类 号：R766.3[医药卫生—耳鼻咽喉科]