建立在PCR-RFLP方法基础上的羊梨形虫的种类鉴定  

Detection and differentiation of the ovine piroplasm species based on the PCR-RFLP method

作　　者：徐宗可[1] 关贵全[1] 刘爱红[1] 马米玲[1] 任巧云[1] 殷宏[1] 罗建勋[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2011年第10期991-994,共4页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30972182)

摘　　要：羊的梨形虫病在世界范围内广泛分布,由多种泰勒虫和巴贝斯虫引起。在我国主要有莫氏巴贝斯虫、羊巴贝斯虫未定种、吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫,给我国畜牧业造成了巨大的经济损失。为了快速准确地鉴定羊梨形虫,作者运用PCR技术扩增了梨形虫的18 S rRNA基因,然后再分别用内切酶ClaⅠ、Sau96Ⅰ和MboⅡ酶切扩增产物,根据酶切后限制性片段多态性的不同,建立了PCR-RFLP方法,达到了鉴定莫氏巴贝斯虫、巴贝斯虫未定种、吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫的目的,为梨形虫的分类和流行病学调查提供了新的方法。Ovine piroplasmosis is an emerging disease worldwide,with multiple species of Babesia ssp. and Theileria spp.now being recognized to infect sheep and goats.Five piroplasms,including B.motasi,B.sp.,T.luwenshuni,T.uilenbergi and T.ovis,have been reported in China,and also caused the great economic losses to stock raising.To develop a taxonomic tool for piroplasm species infective to the sheep and goats,the 18S rRNA genes from all species were amplified using PCR,and digested by Cla Ⅰ,Sau96Ⅰ and Mbo Ⅱ.Results showed that B.motasi,B.sp.,T.luwenshuni,T.uilenbergi and T.ovis can be effectively discriminated based on the restriction fragment length polymorphism（RFLP）.The present study provided a novel assay for molecular identification and taxonomy of piroplasm.

关 键 词：梨形虫 泰勒虫 巴贝斯虫 限制性片段多态性 

分 类 号：S852.723[农业科学—基础兽医学]