鸡胚胎腺垂体糖蛋白激素α亚单位细胞的发育及其变化  

Ontogeny and the developing change of pituitary glycoprotein hormone α subunit(PGHα) cells of chicken embryos

作　　者：何玉琴[1] 刘英[2] 崔胜[3] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]甘肃农业大学动物医学院,甘肃兰州730070 [3]中国农业大学生物学院,北京100094

出　　处：《中国兽医科学》2011年第10期1061-1064,共4页Chinese Veterinary Science

基　　金：国家自然科学基金资助项目(30325034);甘肃省高等学校研究生导师科技项目(110206)

摘　　要：应用免疫组织化学方法,对第3.5～20.5天鸡胚腺垂体糖蛋白激素α亚单位(PGHα)细胞的发生及其在发育过程中的变化规律进行了研究。结果,鸡胚发育的早期(第6.5天),可观察到少量明显的PGHα细胞分布于腺垂体前、后叶,随着胚胎的发育,PGHα细胞数量显著增加(P<0.05),分布于腺垂体前、后叶和结节部,且后叶PGHα细胞数多于前叶。早期PGHα细胞体积小、细胞浆少、细胞核大,随着胚龄的增加,细胞体积增大、细胞浆增多、细胞浆浓染。结果表明,鸡胚腺垂体PGHα细胞发生于胚胎发育的早期,细胞的增殖和分化过程发生在胚胎发育的中后期。The present study was to determine ontogeny of chick embryonic pituitary glycoprotein hormoneα subunit（PGHα cells） and their change during the development of embryos.The pituitary glands were collected on day 3.5 to 20.5 of incubation,respectively.The expression and distribution of pituitary PGHα cells were then detected by the immunohistochemical method.The experimental results demonstrated that scattered and clarity immunopositive PGHα cells were first detected in cephalic lobe and caudal lobe of pituitary gland on day 6.5 of incubation,and then dramatically increased（P0.05） and distributed throughout the whole cephalic lobe,caudal lobe and pars tubercles of pituitary gland during the embryonic periods.In the early stage,the volume of PGHα cells was small with less cytoplasmic and bigger nuclear,and light staining as well;in the mid and later-incubation,the volume of cells became bigger with intensive staining.These results suggested that the ontogeny of PGHα cells is in the early stage,and the proliferation and differentiation of PGHα cells occur during the middle and late stages.

关 键 词：鸡胚胎 腺垂体 垂体糖蛋白激素α亚单位细胞 免疫组织化学 

分 类 号：S852.21[农业科学—基础兽医学]