女贞舒心方对高脂去卵巢大鼠血清E_2水平、动脉管壁ER表达及组织形态学的影响  

Effects of Nüzhenshuxin formula on the level of E2 in serum,the expression of ER in arterial wall and histomorphology with ovariectomized and high fat rats

作　　者：钟栩[1] 王晨[1] 王安春[2] 曾俊杰[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000 [2]甘肃省人民医院

出　　处：《中国老年学杂志》2011年第20期3949-3952,共4页Chinese Journal of Gerontology

基　　金：甘肃省中医药管理局项目(2004-GZK-20)

摘　　要：目的通过观察女贞舒心方(NF)对高脂去卵巢大鼠血清雌二醇(E2)水平、动脉管壁雌激素受体(ER)表达及组织形态学的影响,为本方通过调节雌激素水平而发挥心血管保护作用提供科学依据。方法将成年雌性大鼠60只随机分为5组,分别为正常组(NG)、模型组(MG)、己烯雌酚(DES)组、NF高、低剂量组。除正常组外,切除双侧卵巢,给予高脂饮食,同时给予相应剂量药物灌服,每周测体重,6 w后,测定血清胆固醇(TC)、甘油三酯(TG)、高、低密度脂蛋白胆固醇(HDL-C、LDL-C)含量、血清E2水平及主动脉管壁ER的表达,并观察主动脉管壁形态。结果 NF能减缓大鼠体重增长,降低血清TC、TG、LDL-C水平,升高HDL-C含量,提升血清E2水平,增加动脉管壁ER的表达并能保护动脉管壁,与模型组比较有统计学意义(P<0.05或P<0.01)。结论 NF能调节血脂,增加血清中E2水平,发挥雌激素样作用,对绝经后妇女的血管保护可能是有益的。Objective To observe effect of Nüzhenshuxin formula（NF） on the level of E2 in serum,the expression of ER in arterial wall and histomorphology with ovariectomized and high fat rats.Methods 60 female rats were divided into 5 groups： normal（NG）,model（MG）,Diethylstilbestrol（DES） and NF high,low dose groups.After the models were established successfully,the rats were fed with high fat diet and drug at the same time.The rat′s body weight was recorded once week.6 weeks later,the content of total cholesterol（TC）,triglyceride（TG）,low and high density lipoprotein cholesterol（LDL-C,HDL-C） in serum and the level of E2 and expression of ER in arterial wall were detected,while the artery pipe wall change was observed with microscope.Results After fed with NF for 6 weeks,the weight growth of rats were slowed down,the level of TC,TG and LDL-C were declined,the level of HDL-C was increased,the level of E2 and the expression of ER were raised,while the blood vessel endangium was improved,compared with those of model group（P0.05,P0.01）.Conclusions NF can increase the level of E2 and adjust the blood fat through displaying plant estrogen type function,it is beneficial to blood vessel protection for menopause women.

关 键 词：女贞舒心方 高脂 去势大鼠 植物雌激素 血管保护 

分 类 号：R284[医药卫生—中药学] R589.2[医药卫生—中医学]