雷公藤内酯醇对大鼠大脑皮质内注射Aβ后IL-1α和IL-1β表达的影响  

Effects of triptolide on the expressions of IL-1α and IL-1β after microinjection of beta-amyloid protein into cerebral cortex in rats

作　　者：胡小令[1] 吕诚[1] 杨宝林[1] 万斌[1] 聂菁[1] 李耀斌[1] 

机构地区：[1]南昌大学医学院解剖学教研室,江西南昌330006

出　　处：《中国老年学杂志》2011年第20期3952-3955,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.30660073)

摘　　要：目的探讨雷公藤内酯醇(TP)对大鼠大脑皮质内注射β-淀粉样蛋白(Aβ)后白细胞介素-1α(IL-1α)和白细胞介素-1β(IL-1β)表达的影响。方法 30只SD雄性大鼠等分成对照组、Aβ组、用药组。应用免疫组织化学、RT-PCR方法检测各组大鼠大脑皮质IL-1α和IL-1β的表达。结果免疫组织化学染色显示,用药组IL-1α和IL-1β阳性细胞数较Aβ组明显减少(P<0.05)、平均光密度较Aβ组明显减弱(P<0.01)。RT-PCR显示,用药组IL-1α和IL-1βmRNA水平较Aβ组明显降低(P<0.05,P<0.01)。结论 TP对Aβ所致的大鼠大脑皮质IL-1α和IL-1β的过度表达有抑制作用。Objective To explore the effects of triptolide（TP） on the expression of interleukin-1α（IL-1α） and interleukin-1β（IL-1β） after microinjection of beta-amyloid protein（Aβ） into the cerebral cortex in rats.Methods Thirty male SD rats were equally divided into control,Aβ and TP-treated groups.The variations of IL-1α and IL-1β expressions of the cerebral cortex in these groups were observed by immunohistochemical staining and RT-PCR.Results The cell numbers of IL-1α and IL-1β positive staining in TP-treated group were significantly decreased than those in Aβ group（P0.05）,and the average optical densities of IL-1α and IL-1β positive staining in TP-treated group were also significantly lower（P0.01）.The expression levels of IL-1α and IL-1β mRNA in TP-treated group were significantly lower than those in Aβ group（P0.05,P0.01）.Conclusions TP can inhibit the overexpressions of IL-1α and IL-1β in the cerebral cortex induced by Aβ.

关 键 词：雷公藤内酯醇 Β-淀粉样蛋白 白细胞介素-1Α 白细胞介素-1β 

分 类 号：R285[医药卫生—中药学]