低浓度DNCB诱发豚鼠亚临床接触性皮炎的剂量效应关系  

The Dose-effect of Light Concentration DNCB in Inducing Guinea Pig Sub-clinical Contact Dermatitis

作　　者：李春联[1] 王学民 

机构地区：[1]北京市石景山医院皮肤科,北京100043 [2]上海市皮肤病医院,上海200050

出　　处：《中国皮肤性病学杂志》2011年第11期848-850,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的研究低浓度2,4-二硝基氯苯(DNCB)诱发豚鼠亚临床状态接触性皮炎的剂量-效应关系。方法以0.2000%,0.1000%,0.0500%、0.0250%和0.0125%五种浓度的DNCB溶液连续8周对豚鼠进行暴露,赋形剂作为对照,观察皮肤反应、评分。结果 0.0500%,0.1000%和0.2000%浓度组从第2周起相对于对照组出现有统计学意义的皮肤反应,皮肤反应强度随暴露次数的增多、暴露浓度的增高而增强;其中0.1000%和0.2000%浓度组出现评分>2分的皮肤反应,高于对照组(P<0.05),而0.0500%浓度组出现评分小于2分的亚临床状态皮肤反应,也高于对照组(P<0.05)。豚鼠皮肤反应强度与豚鼠暴露DNCB的累积剂量呈线性正相关(r=0.74,P<0.01)。结论低浓度DNCB可以诱发豚鼠亚临床接触性皮炎反应,反应强度随暴露浓度增高、暴露次数增多而增强;研究为变态反应型敏感性皮肤提供动物实验依据。Objective To evaluate the.dose-effect of light concentration DNCB in inducing guinea pig sub-clinical contact dermatitis. Method The guinea pigs were exposured to 0. 200 0% ,0. 100 0% ,0.050 0% ,0.025 0% and 0.012 5% DNCB solutions for 8 weeks. Excipient were used as control. Skin response was observed and score were obtained. Results Comparing with control group, skin exposured to the 0. 200 0% ,0. 100 0% and 0. 050 0% DNCB showed statistical response from the 2nd week and became more obvious with time-dependent and increased concentrations of the DNCB exposure. The skin responses＇ score in the 0. 200 0% and 0. 100 0% groups was above 2, whinch was higher than those in control （P 〈0.05）. There was a subclinical state of skin response in the 0. 050 0% group, the score was less than 2, which was higher than those in control（ P 〈 O. 05 ）. With the increased dosage of DNCB exposure, there was a linear increase of the skin responses＇ （r =0. 74, P 〈 0.01 ）. Conclusion Low concentration of DNCB can induce guinea pig to suffer from sub-clinical contact dermatitis skin response, and the skin response increases with the time-dependent and concentrations-dependent. Our results could provide animal experiment basis for the allergic sensitive skin.

关 键 词：2 4-二硝基氯苯 豚鼠 接触性皮炎 敏感性皮肤 

分 类 号：R758.22[医药卫生—皮肤病学与性病学] R-332[医药卫生—临床医学]