An α-expansin, VfEXPA1, is involved in regulation of stomatal movement in Vicia faba L.  

作　　者：WEI PengCheng CHEN Su ZHANG XiuQing ZHAO Ping XIONG YanMei WANG WenLong CHEN Jia WANG XueChen 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China [2]Institute of Rice Research, Anhui Academy of Agricultural Sciences, Hefei 230031, China [3]Changde Normal School, Changde 415000, China

出　　处：《Chinese Science Bulletin》2011年第33期3531-3537,共7页

基　　金：supported by the National Basic Research Program of China (2006CB100100, 2003CB114300);the National Natural Science Foundation of China (30370129, 30421002)

摘　　要：The wall loosening of guard cells differs from other types of plant cells. However, the regulation of wall loosening during stomatal movement is poorly understood. VfEXPA1 is an α-expansin gene cloned from Vicia faba epidermal strips. Expression of VfEXPA1 is regulated by darkness and submergence, and is not affected by light and abscisic acid (ABA). In situ hybridization showed that VfEXPA1 is expressed primarily in the guard cells. Overexpression of VfEXPA1 in transgenic tobacco accelerated light-induced stomatal opening, and increased both transpiration and photosynthetic rates under favorable growth conditions. Our results indicate the guard cell-expressed expansin VfEXPA1 plays an important role in regulation of stomatal opening.The wall loosening of guard cells differs from other types of plant cells. However, the regulation of wall loosening during sto- matal movement is poorly understood. VfEXPA1 is an αexpansin gene cloned from Vicia faba epidermal strips. Expression of VfEXPA1 is regulated by darkness and submergence, and is not affected by light and abscisic acid （ABA）. In situ hybridization showed that VfEXPA1 is expressed primarily in the guard cells. Overexpression of VfEXPA1 in transgenic tobacco accelerated light-induced stomatal opening, and increased both transpiration and photosynthetic rates under favorable growth conditions. Our results indicate the guard cell-expressed expansin VfEXPA1 plays an important role in regulation of stomatal opening.

关 键 词：气孔运动 膨大 调控 蚕豆 转基因烟草 保卫细胞 气孔开放 植物细胞 

分 类 号：S643.6[农业科学—蔬菜学]