A vacuole localized β-glucosidase contributes to drought tolerance in Arabidopsis  

作　　者：WANG PengTao LIU Hao HUA HongJie WANG Lei SONG Chun-Peng 

机构地区：[1]State Key Laboratory of Cotton Biology, Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng 475004, China

出　　处：《Chinese Science Bulletin》2011年第33期3538-3546,共9页

基　　金：supported by the National Natural Science Foundation of China (90817106, 31170253 and 30800075)

摘　　要：The phytohormone abscisic acid (ABA) plays a critical role in plant growth, development, and adaptation to various stress conditions. The cellular ABA level is constantly adjusted to respond to changing physiological and environmental conditions. To date, the mechanisms for fine-tuning ABA levels remain elusive. Here, we report that BGLU10, a member of a multigene family of β-glucosidases, contributes to drought tolerance in Arabidopsis. The T-DNA insertion mutant bglu10 exhibited a droughtsensitive phenotype, characterized by an increased rate of water loss, and lower leaf temperature, β-glucosidase activity, ABA content, and expressions of ABA-and drought-responsive genes under drought stress. In contrast, lines overexpressing BGLU10 showed greater drought resistance than that of the wild-type, as shown by decreased water loss via transpiration, higher β-glucosidase activity, ABA level, and expressions of ABA- and stress-responsive genes under drought stress. Transient expression of BGLU10::GFP and γ-TIP1::RFP in mesophyll cell protoplasts showed that the BGLU10 enzyme protein was localized to the vacuole. Meanwhile, BGLU10 was expressed in various organs, and was induced by several abiotic stresses, suggesting that BGLU10 may be involved in a variety of stress responses, and that hydrolysis of ABA-GE produces free ABA in the plant stress response.The phytohormone abscisic acid （ABA） plays a critical role in plant growth, development, and adaptation to various stress conditions. The cellular ABA level is constantly adjusted to respond to changing physiological and environmental conditions. To date, the mechanisms for fine-tuning ABA levels remain elusive. Here, we report that BGLU10, a member of a multigene family of β-glucosidases, contributes to drought tolerance in Arabidopsis. The T-DNA insertion mutant bglulO exhibited a drought- sensitive phenotype, characterized by an increased rate of water loss, and lower leaf temperature, β-glucosidase activity, ABA content, and expressions of ABA-and drought-responsive genes under drought stress. In contrast, lines overexpressing BGLUIO showed greater drought resistance than that of the wild-type, as shown by decreased water loss via transpiration, higher D-glucosidase activity, ABA level, and expressions of ABA- and stress-responsive genes under drought stress. Transient expression of B GLU10：：GFP and γ-TIP I：：RFP in me sophyll cell protoplasts showed that the B GLU 10 enzyme protein was localized to the vacuole. Meanwhile, BGLUIO was expressed in various organs, and was induced by several abiotic stresses, suggesting that BGLU10 may be involved in a variety of stress responses, and that hydrolysis of ABA-GE produces free ABA in the plant stress response

关 键 词：葡萄糖苷酶 抗旱性 拟南芥 液泡 ABA含量 本地化 多基因家族 干旱胁迫 

分 类 号：Q946.5[生物学—植物学]