氧化苦参碱对大鼠局灶性脑缺血再灌注损伤BDNF及其受体TrkB表达的影响  

Effect of oxymatrine on the expression of BDNF and its receptor TrkB after focal cerebral ischemia-reperfusion in rats

作　　者：付燕燕[1] 段静雨[2] 段昌柱[3] 

机构地区：[1]徐州医学院生物学教研室,徐州221004 [2]徐州医学院中药学教研室,徐州221004 [3]重庆医科大学基础医学院生物学教研室,重庆400016

出　　处：《重庆医科大学学报》2011年第9期1047-1050,共4页Journal of Chongqing Medical University

摘　　要：目的:观察氧化苦参碱(Oxymatrine,OMT)对大鼠局灶性脑缺血再灌注后皮质脑源性神经生长因子(Brain-derivedneurotrophic factor,BDNF)及其受体酪氨酸激酶B(Tyrosine kinase B,TrkB)表达的影响,探讨OMT神经保护作用的机制。方法:采用线栓法建立大鼠大脑中动脉缺血再灌注模型。SD雄性大鼠随机分为假手术组、模型组、阳性药尼莫地平组和OMT低、中、高剂量给药组6组,缺血2 h再灌注24 h后进行神经功能评分。免疫组化法检测BDNF阳性细胞,Western blot检测BDNF和TrkB蛋白表达。结果:与模型组比,OMT中、高剂量组神经学评分均减低,BDNF阳性细胞数以及BDNF和其受体TrkB蛋白表达显著增加,差异有统计学意义(P<0.05,P<0.01)。结论:OMT对大鼠局灶性脑缺血再灌注损伤的恢复有一定促进作用,其机制可能与上调BDNF及受体TrkB的表达有关。Objective：To study the effects of oxymatrine（OMT） on brain-derived neurotrophic factor（BDNF） and its receptor tyrosine kinase（TrkB） in cerebral cortex after focal cerebral ischemia-reperfusion in rats,and explore the neuroprotective mechanism of oxymatrine.Methods：The rat middle cerebral artery occlusion models with filament of focal cerebral ischemia-reperfusion were established.SD male rats were divided randomly into sham group,cerebral ischemia-reperfusion model group,nimodipine（Nim） group and the oxymatrine group.the oxymatrine group was futher divided into three sub-groups which were dealt with the low,middle and high dose of oxymatrine.The neurological scores were used after 2 h ischemia followed by 24 h reperfusion.The expression of BDNF positive neurons,BDNF and TrkB proteins were respectively detected by immunohistochemistry and western blot.Results：Compared with the sham group,the neurological scores decreased,while the expression of BDNF positive neurons,BDNF and its receptor TrkB protein significantly increased in middle and high dose of oxymatrine groups（P0.05;P0.01）.Conclusion：OMT protects the rats from the ischemia injury following focal cerebral ischemia-reperfusion,the mechanism of which may partly be due to up-regulating the expression of BDNF and TrkB.

关 键 词：氧化苦参碱 脑缺血再灌注 脑源性神经生长因子 酪氨酸激酶B 

分 类 号：R285.5[医药卫生—中药学] R743.31[医药卫生—中医学]