支气管哮喘患儿诱导痰IL-17水平分析  

Research on the concentrations of interleukin-17 in induced sputum of patients with asthma

作　　者：高惠[1] 罗征秀[1] 刘超[1] 任洛[1] 蒋永惠[1] 王莉佳[1] 刘恩梅[1] 符州[1] 

机构地区：[1]重庆医科大学附属儿童医院呼吸中心,重庆400014

出　　处：《重庆医科大学学报》2011年第9期1094-1096,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:81070015);重庆市科委基金资助项目(编号:CSTC2009BB5258);重庆市卫生局基金资助项目[渝卫科教(2009)66号-2009-2-241]

摘　　要：目的:分析儿童支气管哮喘急性发作期、慢性持续期及临床缓解期诱导痰上清液白细胞介素-17I(nterleukin 17I,L-17)、白细胞介素-6I(nterleukin 6I,L-6)、转化生长因子-β(Transforming growth factor-β,TGF-β)水平,探讨其在哮喘发病过程中的作用。方法:收集支气管哮喘急性发作期25例、慢性持续期15例、缓解期17例及健康对照儿童13例诱导痰,采用ELISA法测定诱导痰上清液IL-17I、L-6及TGF-β水平,比较各组细胞因子水平差异。结果:哮喘患儿急性发作期诱导痰上清液IL-17浓度为(855.39±46.89)pg/ml,慢性持续期为(705.63±46.29)pg/ml,临床缓解期为(597.09±60.26)pg/ml,健康对照儿童为(434.12±43.63)pg/ml,哮喘患儿诱导痰上清液IL-17水平显著高于健康对照儿童,差异具有统计学意义(P<0.05),且哮喘急性发作期IL-17水平显著高于慢性持续期、缓解期,差异具有统计学意义(P<0.05);哮喘患儿急性期IL-6水平明显高于其他各组,差异有统计学意义(P<0.05);各组TGF-β水平差异无统计学意义(P>0.05)。结论:诱导痰上清液IL-6I、L-17表达增加在哮喘发病中有重要作用。Objective：To investigate the changes of IL-17,TGF-β and IL-6 concentrations in the induced sputum of asthma patients of different stages.Methods：Sputum IL-17,TGF-β and IL-6 concentrations were measured in 57 patients with asthma including 25 in acute stage,15 in chronic stage,and 17 in remission stage,and another 13 healthy children were in the control group.They were measured with sandwich enzyme immunosorbent assay（ELISA）.Results：The IL-17 concentration in different stages was as follows：（855.39±46.89） pg/ml in acute stage,（705.63±46.29） pg/ml in chronic stage,and（597.09±60.26） pg/ml in remission stage,the IL-17 concentration in healthy control group was（434.12±43.63） pg/ml.The mean IL-17 concentration was increased in asthma compared with healthy control group（P〈0.05）.The IL-17 and IL-6 concentrations in acute stage were significantly higher than in chronic and remission stage（P〈0.05）.TGF-β concentration was measured in the different stages,and there were no significantly differences between them.Conclusion：IL-17 and IL-6 concentrations are significantly increased in asthma and may be used as one of the indices of airway inflammation.

关 键 词：支气管哮喘 诱导痰 白细胞介素-17 白细胞介素-6 转化生长因子-Β 

分 类 号：R725.6[医药卫生—儿科]