甘蔗SPSⅢ5′侧翼序列缺失表达载体的构建及其转化烟草的研究  

Construction of SPS Ⅲ in Sugarcane 5′ Flank Sequence Deletion Expression Vectors and the Transformation in Tobacco

作　　者：池朝华[1,2] 陈玲[1,2] 周平[3] 高玉娜[1,2] 叶冰莹[1,2] 陈由强[1,2] 

机构地区：[1]福建师范大学生命科学学院,福州350108 [2]农业部甘蔗遗传改良重点开放实验室,福州350108 [3]福建省农业科学院果树研究所,福州350013

出　　处：《植物研究》2011年第6期696-701,共6页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(30871575);国家现代农业技术体系建设专项(CARS-20)

摘　　要：蔗糖磷酸合成酶是糖代谢调节的关键酶之一,根据本实验室克隆到的SPSⅢ5′侧翼序列,构建了3个侧翼序列缺失表达载体,转化云烟85成功获得转基因植株,可用于核心启动子确定的研究。转基因植株叶片冰冻切片结果表明3段缺失侧翼序列均有启动子活性。Sucrose phosphate synthase is one of the key enzymes in the metabolism of sucrose. Based on the 5＇ flank sequence of SPS Ⅲ cloned in our lab, 3 flank sequence deletion expression vectors were constructed and transformed in YUNYAN 85 tobacco leaves. Three transgenic plants were obtained, which can be used in the identification of core promoter. The results revealed that all these three deletion flank sequences have promoter activity.

关 键 词：蔗糖磷酸合成酶Ⅲ 核心启动子 绿色荧光蛋白 

分 类 号：S566.1[农业科学—作物学]