系统性硬皮病患者外周血CD4^＋T细胞中CD40L表达水平的研究  

Expression of CD40L in CD4 ^＋ T cells from patients with systemic sclerosis

作　　者：廉晓日[1] 胡新红[1] 肖嵘[1] 金藏拓郎[3] 姜红岩[1] 杨艳[1] 王瑶瑶[1] 李亚萍[1] 张桂英[1] 赵明 陆前进 

机构地区：[1]中南大学湘雅二医院皮肤科,长沙410011 [2]医学表观基因组学湖南省重点实验室 [3]鹿儿岛大学医学部

出　　处：《中国医师杂志》2011年第10期1297-1300,共4页Journal of Chinese Physician

基　　金：国家自然科学基金（30972662）,科技部国际科技合作重点项目（2008DFA31980）

摘　　要：目的研究系统性硬皮病（SSc）患者外周血CD4^＋T细胞中CIM0配体（CIMOL）的表达水平。方法应用密度梯度离心法分离26例SSc患者（女16例，男10例）和25例正常对照者（女15例，男10例）的外周血单个核细胞，磁珠分选CD4^＋T细胞。RT—PCR检测CD4^＋T细胞中CD40L mRNA的表达水平；流式细胞术检测CD40L蛋白在CD4^＋T细胞中的表达水平。结果SSc女性患者CD4^＋T细胞中CD40L mRNA和CD40L蛋白的表达水平均显著高于正常女性对照组[5．61±1．86vs2．80±0．94，P〈0．01；（6．70±3．55）％vs（2．37±1．39）％，P〈0．05]；SSc男性患者CD40L mRNA和CD40L蛋白的表达水平与正常男性对照组相比差异均无统计学意义[2．59±0．89vs1．92±0．56，P〉0．05；（2．06±1．09）％vs（2．13±0．87）％，P〉0．05]。结论女性SSc患者CD^＋T细胞中CD40L表达显著增高，而男性患者无明显改变，提示CD40L仅在女性SSc发病中起着重要的作用。Objective To investigate the expression levels of CD40L in CD4^＋ T cells from systemic sclerosis （SSc） patients. Methods PBMC （peripheral blood mononuelear cells） cells were isolated from the peripheral venous blood of SSc patients （16females, 10males） and healthy donors （15females, 10males） by density gradient centrifugation. CD4^ ＋ T cells were isolated using magnetic beads, mRNA levels of CD40L in CD4 ^＋ T cells were measured by real-time quantitative polymerase chain reaction （ RT- PCR）. Flow cytometric analysis was used to detect the CD40L protein on the surface of CD4^ ＋ T cells. Resuits Both CD40L mRNA and protein expression in CD4 ^＋T cells was significantly elevated in female SSe patients compared with female healthy controls [ 5.61 ± 1.86 vs 2. 80 ± 0. 94, P 〈 0.01 ; （6. 70 ± 3.55 ） % vs （2. 37 ± 1.39）%, P 〈0. 05, respectively]. No significant increase in CD40L mRNA and protein expression was observed in male SSc patients compared with male controls [ 2. 59 ± 0. 89 vs 1.92 ± 0. 56, P 〉 0. 05 ; （2. 06 ± 1.09）% vs （2. 13 ±0. 87）%, P 〉0. 05, respectively]. Conclusions CD40L was overexpressed in CD4 ＋ T cell of female patients but not males, which maybe one of the important reasons for female susceptibility to SSc.

关 键 词：硬皮病 系统性/代谢 CD40配体/代谢 CD4阳性T淋巴细胞 

分 类 号：R593.25[医药卫生—内科学]