50种中草药体外抑杀嗜水气单胞菌的药效研究  

Bacteriostasis of 50 traditional Chinese medicine herbs against Aeromonas hydrophila in vitro

作　　者：陈霞[1] 张其中[1,2] 李春涛[1] 

机构地区：[1]西南大学生命科学学院水产科学重庆市市级重点实验室/三峡库区生态环境教育部重点实验室,重庆400715 [2]暨南大学水生生物研究所,广东广州510632

出　　处：《中国预防兽医学报》2011年第11期862-865,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家十二五863计划项目(2011AA10A216);重庆市科技攻关计划项目(CSTC;2010AC1116);广东省海洋与渔业局科技推广项目(A201001H01);广州市科技支撑计划项目(2008Z1-E141)

摘　　要：为筛选有效抑制嗜水气单胞菌(A.hydrophila)的中草药,以期为水产养殖业中防治该菌引起的疾病提供实验依据。本研究采用琼脂扩散法(打孔法)测定了地榆等50种中草药对A.hydrophila的抑制作用,并用二倍稀释法测定抑菌效果较强的25种中草药对A.hydrophila的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果显示地榆的抑菌效果最好,其抑菌圈直径达20 mm以上,MIC为3.91 mg/mL,MBC为7.81 mg/mL;黄连等24种中草药有一定的抑菌和杀菌效果,巴戟天等25种中草药无抑菌作用。结果表明地榆对A.hydrophila的体外抑制效果最好。To evaluate the bacteriostasis of traditional Chinese herbs against Aeromonas hydrophila,50 kinds of herbs were tested by diffusion method（punch method） for bacteria inhibitory and the double dilution method for the minimal inhibitory concentration（MIC） and the minimum bactericidal concentration（MBC）.The results showed that Sanguisorba officinalis had the best inhibitory effect on the bacteria with the inhibitory diameter exceeded 20 mm,meanwhile,the MIC was 3.91 mg/mL and MBC was 7.81 mg/mL;24 kinds of herbs including Coptis chinensis had moderate bacteriostatic effects.On the contrary,the other 25 kinds of herbs including Morinda officinalis had no any inhibitory effect on A.hydrophila.The results indicated that the most effective Chinese herbs against A.hydrophila was Sanguisorba officinalis.

关 键 词：嗜水气单胞菌 中草药 抑菌效果 

分 类 号：S853.7[农业科学—临床兽医学]