糖尿病肾病大鼠肾组织α-actinin-4表达及通络泄浊方的干预作用  

Expression of α-actinin-4,Effects of Tongluoxiezhuofang Intervention in Rats with Diabetic Nephropathy

作　　者：李思佳[1] 彭文[1] 王浩[1] 付文成[1] 王云满[1] 刘育军[1] 

机构地区：[1]上海中医药大学附属普陀医院肾内科,上海200062

出　　处：《中国中西医结合肾病杂志》2011年第10期856-859,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：上海市教委基金资助项目(No.09YZ133);上海高校创新团队建设项目

摘　　要：目的:探讨α-actinin-4在糖尿病肾病大鼠肾组织中表达的变化,并观察通络泄浊方对其表达的影响。方法:采用链脲佐菌素(STZ)法制作大鼠模型,随机分为正常对照组、模型组、通络泄浊方组、科素亚组和通络泄浊方+科素亚组。采用Realtime-PCR及Westernblot方法检测肾组织α-actinin-4mRNA和蛋白的表达。结果:与正常对照组相比,模型组mRNA表达和蛋白明显降低(P<0.01),给药组与模型组相比,mRNA和蛋白表达明显增高(P<0.01)。结论:α-acti-nin-4的表达降低参与了糖尿病肾病大鼠蛋白尿的发生;通络泄浊方可以通过上调DN大鼠肾组织α-actinin-4mRNA和蛋白的表达而改善大鼠的肾功能及蛋白尿水平。Objective：To investigate α-actinin-4 expression and the effects of Tongluoxiezhuofang intervention in rats with diabetes nephropathy.Methods：streptozotocin（STZ）-induced diabetic rats were established,rats were randomized into five groups：the normal group,model group,Tongluoxiezhuofang treatment group,losartan treatment group,Tongluoxiezhuofang combined losartan treatment group.Realtime-PCR and Westernblot were employed to study the change of α-actinin-4 mRNA and protein.Results：The level of expression of α-actinin-4 mRNA and protein in the model group were less than that in the normal group markedly（P〈0.01）,but the level of expression in treatment group were more than the model group（P〈0.01）.Conclusion：There was close relationship between the expression of α-actinin-4 with diabetic nephropathy and urine protein.It is initially confirmed that Tongluoxiezhuofang can up-regulate the expression of α-actinin-4 and reduce the urine protein on diabetic nephropathy rats.

关 键 词：糖尿病肾病 Α-ACTININ-4 通络泄浊方 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]