新疆和田地区人群血清抗HEV水平调查  

Investigation on serum levels of anti-HEV in the population in Hetian district of Xinjiang province

作　　者：钟崇芳[1] 李俊红[1] 胡志明 袁春鸽 王燕 郝娃[1] 牛京勤[1] 殷继明[1] 严艳[1] 李卓[1] 王佑春 

机构地区：[1]首都医科大学附属北京佑安医院佑安医疗联盟,100069 [2]新疆和田地区传染病专科医院肝病科,和田848000 [3]新疆和田市人民医院检验科,和田848000 [4]中国药品生物制品鉴定所细胞室,北京100050

出　　处：《传染病信息》2011年第5期289-292,共4页Infectious Disease Information

基　　金：国家高技术研究发展计划(863计划)项目(2006AA02Z453)

摘　　要：目的 调查新疆维吾尔自治区（新疆）和田地区在1986-1989年戊型肝炎流行后,目前人群中血清抗戊型肝炎病毒（hepatitis E virus,HEV）特异性抗体水平及当地HEV感染的状态.方法 应用酶联免疫方法检测抗HEV IgG和抗HEVIgM,检测新疆和田地区1～90岁健康人血清3070例,对抗HEV IgM阳性血清用实时荧光PCR方法检测HEV RNA,酶联免疫方法检测HEV Ag.结果 3070例血清中维吾尔族者2627例,汉族者443例.检测结果显示抗HEV IgG阳性1074例,总阳性率为34.98%；维吾尔族人群感染率为34.64%,汉族人群感染率为37.18%,2组人群血清抗HEV阳性率差异无统计学意义.抗HEV lgM与抗HEV IgG抗体2项均为阳性20例,单纯抗HEV IgM阳性7例；实时荧光PCR检测HEV RNA均为阴性,未见临床感染患者.不同年龄组抗HEV IgG存在差异,基本上呈现随着年龄的增长,人群抗体水平缓慢上升的趋势.结论 新疆和田地区人群中仍然有HEV感染存在,但是主要表现为亚临床感染.Objective To investigate the current status of specific anti-HEV antibodies and HEV infection in Hetian district of Xinjiang province after hepatitis E outbreak occurred in the region from 1986 to 1988. Methods Totally 3070 serum samples were obtained from healthy subjects at the age of 1-90 in Hetian district of Xinjiang province. HEV IgG and IgM were detected by enzyme-linked immunosorbent assay （ELISA） and HEV RNA was detected by real-time fluorescent RT-PCR and HEV-Ag by ELISA in the serum samples with HEV IgM positive. Results Among 3070 serum samples, 2627 were obtained from Uyghur people and 443 from Han people. The results showed that 1074 samples （34.98%） were HEV IgG positive, and the infective rate was 34.64% in Uyghur people and 37.18% in Han people. Anti-HEV positive rate was not significantly different between Uyghur people and Han people. Twenty samples were both HEV IgM and IgG positive, and 7 were only HEV IgM positive. HEV RNA was nega- tive in all the subjects by real-time fluorescent RT-PCR, and no infected patients were found. HEV IgG was significantly different among the subjects at different ages, and antibody levels rose slowly with age. Conclusion There are still HEV infections among healthy people in Hetian district of Xinjiang province, mainly manifested with sub-clinical infection.

关 键 词：戊型肝炎病毒 抗体 流行病学 

分 类 号：R512.6[医药卫生—内科学] R18[医药卫生—临床医学]