人疱疹病毒8型ORF26单核苷酸多态性研究  

Single nucleotide polymorphisms in the open reading frame 26 （ORF26） gene of human herpesvirus 8 （HHV-8） in Kaposi＇s sarcoma

作　　者：吴秀娟[1] 石岩[1] 靳颖[1] 张德志[1] 普雄明[1] 

机构地区：[1]新疆维吾尔自治区人民医院皮肤性病科,乌鲁木齐830001

出　　处：《中华皮肤科杂志》2011年第11期805-807,共3页Chinese Journal of Dermatology

基　　金：新疆维吾尔自治区自然科学基金（2009211A24）

摘　　要：目的探讨人疱疹病毒8型（HHV-8）ORF26的单核苷酸多态性，分析其与Kaposi肉瘤不同临床分型及黏膜侵袭性的相关性。方法Kaposi肉瘤患者32例，其中经典型26例，艾滋病相关型6例。使用酚-氯仿-异戊醇方法对Kaposi肉瘤石蜡包埋组织进行HHV-8 DNA抽提，采用巢式PCR方法扩增ORF26基因并双向测序，使用DNAStar软件和Clustal W软件分析ORF26基因的单核苷酸多态性。运用Fisher确切概率法对结果进行统计学分析。结果ORF26基因研究发现，32例Kaposi肉瘤患者中HHV-8阳性30例，6例艾滋病相关型HHV-8均为阳性。30例患者的病毒株中，HHV-8 ORF26基因SNP主要集中在981T／C（12例）、1086C／T（12例）、1139A／C（12例）；HHV-8 ORF26基因单核苷酸多态性在不同临床分型或有无黏膜损害的Kaposi肉瘤之间的差异无统计学意义。结论HHV-8 ORF26基因单核苷酸多态性可能与Kaposi肉瘤不同临床分型和黏膜侵袭性无关。Objective To study the single nucleotide polymorphisms （SNPs） in the ORF26 gene of HHV-8 in Kaposi＇s sarcoma （KS）, and to assess their correlations with the clinical phenotype and mucosal invasion of KS. Methods HHV-8 DNA was extracted with phenol-chloroform-isoamyl alcohol from paraffin-era- bedded tissue specimens obtained from 32 cases of KS ORF26 gene of HHV-8 was amplified by nested-PCR （including 26 classic and 6 AIDS-related KS）. The followed by bidirectional sequencing. The software DNAStar and program Clustal W were used to assess the SNPs in the ORF26 gene. Statistical analysis was carried out by using the Fisher＇s exact probability test. Results HHV-8 DNA was detected in 30 of the 32 tissue specimens, and in all of the 6 AIDS-related specimens. The predominant SNPs were 981 T/C （n = 12）, 1086 C/T （n = 12） and 1139 A/C （n = 12） in the ORF26 gene of the 30 strains of HHV-8. No significant difference was observed in the distribution of SNPs in ORF26 between different phenotypes of KS or between KS with and without mucosal invasion. Conclusion The ORF26 SNPs of HHV-8 seem unrelated to the clinical phenotypes or mucosal invasion of KS.

关 键 词：单核苷酸多态性 人疱疹病毒8型 KAPOSI肉瘤 HHV-8 巢式PCR方法 26基因 统计学分析 临床分型 

分 类 号：R51[医药卫生—内科学]