类风湿关节炎活动期滑液中葡萄糖6-磷酸异构酶水平及与抗环瓜氨酸肽抗体的关系  

The level of glucose-6-phosphate isomerase in synovial fluid of active rheumatoid arthritis and its correlation with anti-cyclic citruliine peptide antibody

作　　者：刘荣清[1] 孙伯坚[3] 李亚平[2] 林佳静[3] 李海波[1] 韩梅[3] 

机构地区：[1]宁夏医科大学总医院风湿免疫科,银川750004 [2]宁夏医科大学总医院骨三科,银川750004 [3]宁夏医科大学基础医学院病原生物学与医学免疫学系

出　　处：《中华风湿病学杂志》2011年第11期739-741,共3页Chinese Journal of Rheumatology

基　　金：基金项目：宁夏自然科学基金（NZ09107）

摘　　要：目的检测葡萄糖-6-磷酸异构酶（GPI）在类风湿关节炎（RA）活动期膝关节滑液中的含量，并探讨滑液中GPI与抗环瓜氨酸肽（CCP）抗体的关系。方法用酶联免疫吸附法（ELISA）检测22例RA活动期患者和37例骨关节炎活动期患者滑液中GPI和抗CCP抗体的浓度。组间比较采用t检验，相关性分析采用Spearman相关分析。结果RA活动期患者膝关节滑液中的GPI浓度高于骨关节炎活动期患者[（9．6±8_4）和（0．9±1．8）μg／ml]，差异有统计学意义（P〈0．01）；RA活动期患者与骨关节炎活动期患者滑液的抗CCP抗体浓度分别为（14．61±18．64）和（1．42±0．09）U／ml，差异有统计学意义（P〈0．01）；RA活动期患者膝关节滑液中GPI含量和抗CCP抗体浓度呈正相关（r=0．447，P=0．037）。结论GPI在RA活动期患者滑液中高表达，可能与RA发生发展过程中慢性滑膜炎、骨质破坏有关。GPI与抗CCP抗体呈正相关，两者可作为RA实验室诊断的参考指标。Objective To detect glucose-6-phosphate isomerase （GPI） in knee joint synovial fluid of active rheumatoid arthritis （RA） and explore the correlation between GPI and anti-cyclic citrulline peptide antibody （anti-CCP）. Methods GPI and anti-CCP in the synovial fluid were measured by enzyme linked immunosorbent assay （ELISA） from 22 patients with active RA and 37 patients with active osteoarthritis （OA）. Student＇s t-test was used for intergroup comparison and Spearman＇s analysis was used for correlation analysis. Results The level of GPI and anti-CCP in the synovial of active RA [（9.6±8.4） μg/ml, （14.61±18.64） U/ml] was significantly higher than that of active OA[ （0.9±1.8） μg/ml, （1.42±0.09） U/ml）]. There was positive correlation between GPI and anti-CCP （r=0.447, P=-0.037）. Conclusion High expression of GPI is shown in active RA synovial fluid. It is suggested that GPI as an antigen that may participate in chronic synovitis, bone destruction and joint malformation. Both GPI and anti-CCP may be the laboratory markers for the diagnosis of RA.

关 键 词：关节炎 类风湿 葡糖-6-磷酸异构酶 滑液 抗环瓜氨酸肽抗体 

分 类 号：R593.22[医药卫生—内科学]