筋脉通含药血清对高糖培养施万细胞8-羟基脱氧鸟苷和活化的caspase-3表达的影响  

Effects of Medicated Serum Containing Jinmaitong on 8-OHdG and Active Caspase-3 of Schwann Cells in High Glucose Medium

作　　者：朴元林[1] 梁晓春[1] 赵丽[1] 张宏[2] 李伯武[1] 黄文智[1] 

机构地区：[1]中国医学科学院/北京协和医学院北京协和医院中医科/协和转化医学中心/卫生部内分泌重点实验室,100730 [2]中国医学科学院/北京协和医学院基础医学研究所细胞中心,100005

出　　处：《医学研究杂志》2011年第10期35-39,共5页Journal of Medical Research

基　　金：北京市自然科学基金资助项目(7082077)

摘　　要：目的探讨筋脉通含药血清对高糖培养施万细胞8-羟基脱氧鸟苷(8-hydoxydeoxyguanosine,8-OHdG)水平及活化的半胱氨酸天冬氨酸酶3(cysteine aspartase-3,caspase-3,)(17kDa)蛋白及mRNA表达的影响。方法将体外培养的施万细胞分为高糖组、筋脉通组(加入筋脉通含药血清)、维生素C组(加入维生素C含药血清)及正常对照组,采用酶联免疫吸附法检测施万细胞上清液中8-OHdG的分泌量,免疫荧光法检测活化的caspase-3(17kDa)蛋白表达,实时荧光定量PCR法检测活化的caspase-3(17kDa)mRNA的表达。结果与正常对照组比较,高糖培养施万细胞上清液中8-OHdG的分泌量及细胞内活化的caspase-3(17kDa)蛋白和mRNA表达均明显升高(P<0.01);与高糖组比较,筋脉通组细胞上清液中8-OHdG的分泌量及细胞内活化的caspase-3(17kDa)蛋白和mRNA表达明显降低(P<0.01)。结论筋脉通含药血清可改善高糖导致的施万细胞DNA氧化损伤和细胞凋亡,提示筋脉通可能改善糖尿病神经病变之氧化损伤及细胞凋亡。Objective To investigate the effects of medicated serum containing Jinmaitong （JMT） on the secretion level of 8 - OHdG and the expression of active caspase- 3（ 17kDa）protein and mRNA of Schwann cells（SCs） in high glucose medium. Methods Cultured SCs were divided into high - glucose group, JMT group （ adding JMT - containing serum） , vitamin C group （ adding vitamin C - containing serum） and normal control group. The concentration of 8 - OHdG in the superuatant of cultured SCs was detected by enzyme - linked immunosorbent assay. The expression of active caspase - 3 （17kDa） protein was detected by immunofluorescence. The expression of active caspase - 3 mRNA in SCs was detected by real - time fluorescence quantitative PCR. Results Compared with normal control group, the secretion level of 8 -OHdG in the supernatant and the expression of the intracellular active caspase -3 （17kDa） protein and mRNA were significantly increased in high - glucose group （ P 〈 0.01 ） ; Compared with high - glucose group, the secretion level of 8 - OHdG in the supernatant and the expression of the intracellular active caspase - 3 （17kDa） protein and mRNA were significantly decreased in JMT group （P 〈 0.01 ）. Conclusion The medicated serum containing JMT can improve high - glucose induced oxidative injury of DNA and apoptosis in SCs, suggesting JMT might improve oxidative injury and apoptosis in diabetic neuropathy.

关 键 词：施万细胞 8-羟基脱氧鸟苷 caspase-3 筋脉通 

分 类 号：R651.15[医药卫生—外科学]