穿膜融合多肽TAT-N24对胃癌细胞迁移的影响  

Influence of Cell-permeable TAT-N24 Fusion Peptide on Cell Migration of Gastric Carcinoma Cells

作　　者：刘秀财[1] 郭红艳[1] 高涵[1] 齐晓丹[1] 兴桂华[1] 王淑英[1] 孙晓杰[1] 

机构地区：[1]齐齐哈尔医学院生物化学教研室,161006

出　　处：《医学研究杂志》2011年第10期69-71,共3页Journal of Medical Research

基　　金：黑龙江省教育厅面上项目课题(11531431);齐齐哈尔市科技局指导项目

摘　　要：目的观察穿膜融合多肽TAT-N24对胃癌细胞MGC803迁移的影响。方法用纯化的融合多肽TAT-N24处理胃癌MGC803细胞,通过细胞迁移实验检测其对细胞运动迁移能力的影响,明胶酶谱实验观察其对肿瘤转移相关基因MMP9表达的影响。结果经TAT-N24多肽处理的MGC803细胞,其细胞迁移能力明显低于对照组细胞,差异具有统计学意义(P<0.05),但其对肿瘤转移相关基因MMP9的表达和分泌却没有影响。结论融合多肽TAT-N24能够抑制胃癌MGC803细胞的运动迁移能力,其在胃癌的治疗上可能具有潜在的应用前景。Objective To observe the effects of cell - permeable TAT - N24 fusion peptide derived from p553, regulatory subunit of pbosphoinositide -3 kinase on cell migration of MGCS03 gastric carcinoma cells. Methods Gastric cancer cells MGC803 was treated with the purified fusion peptide TAT - N24. The ability of cell migration was detected by transwell migration assay. Expression of tumor metastasis associated gene MMP9 was observed by gelatin zymography. Results TAT - N24 fusion protein markedly resulted in decrease of the MGC803 cell migration compared with the control cells （P 〈 0.05 ）. But it did not affect the expression and secretion of MMP9. Conclusion TAT - N24 fusion peptide could inhibit cell migration in gastric carcinoma MGC803 cells. It perhaps has potential clinical application in gastric carcinoma.

关 键 词：TAT—N24 胃癌 细胞迁移 磷脂酰肌醇3-激酶(PI3K) 

分 类 号：R977.21[医药卫生—药品]