赛庚啶对大鼠脑缺血再灌注损伤的保护作用及对神经细胞凋亡的影响  

Effects of Cyproheptadine on cerebral ischemia-reperfusion injury and nerve cell apoptosis

作　　者：谭婷[1] 王美纳[2] 马小亚[2] 

机构地区：[1]西安交通大学医学院第二附属医院科研部 [2]西安交通大学医学院第二附属医院药剂科

出　　处：《中国临床药理学与治疗学》2011年第10期1096-1100,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:探讨赛庚啶(Cyproheptadine,CYP)对大鼠局灶性脑缺血再灌注损伤的保护作用及机制。方法:利用Longa线栓法制作大鼠局灶性脑缺血3h再灌注3、6、24、48h模型,TTC染色观察脑梗死体积,图像分析系统测定脑梗死范围,紫外分光光度仪测定血清乳酸脱氢酶(LDH)含量,TUNEL法检测凋亡神经细胞,免疫组化法测定脑细胞Caspase-3的表达。结果:与模型组比较,CYPI、II组的脑梗死绝对容积及梗死百分比显著减小(P<0.01),由于缺血再灌注导致的血清LDH含量升高显著降低(P<0.01),凋亡神经细胞及Caspase-3阳性表达产物明显减少(P<0.01)。结论:CYP对大鼠脑缺血再灌注损伤具有保护作用,抑制神经细胞凋亡过程,其机制与CYP抑制血清LDH升高及减少Caspase-3表达有关。AIM：To study the protection and mechanism of Cyproheptadine（CYP） on focal cerebral ischemia-reperfusion in rats. METHODS：The models with cerebral ischemicre-reperfusion were induced by intraluminal middle cerebral artery occlusion（MCAO） with a nylon monofilament suture,2 h occlusion followed by 3,6,24,48 h reperfusion.The volume of cerbral infarction was observed using 2,3,4-triphenyltetrazolium chloride（TTC） dyeing,apoptosis of the neurocytes was detected by TUNEL technique,the expression levels of Caspase-3 proteinum in brain tissue were detected by immunohistochemistry technique. RESULTS：Compared with model group,the volumes of cerbral infarction were reduced significantly in the CYPI,IIgroups（P0.01）,the increased LDH had been decreased（P0.01）,apoptosis of the neurocytes of ischemia area in the brain tissue was depressed by CPY,the ratio of Caspase-3 proteinum expression was decreased（P0.01）. CONCLUSION：The CPY can prevent cerebral ischemia reperfusion injury by reduce the number of nerve cells apoptosis and the level of Caspase-3 in brain.

关 键 词：赛庚啶 脑缺血再灌注损伤 保护作用 神经细胞凋亡 CASPASE-3 

分 类 号：R965.2[医药卫生—药理学]