蝙蝠葛碱对离体豚鼠气管平滑肌舒张作用的研究  

Relaxation effects of dauricine on isolated tracheal smooth muscles of guinea pigs

作　　者：沈柏儒[1] 曾志林[1] 许继德[1] 刘思[1] 许小洋[1] 朱伟思[1] 李茵[1] 简文华[1] 

机构地区：[1]广州医学院生理教研室

出　　处：《中国临床药理学与治疗学》2011年第10期1110-1113,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：广州市高校科技项目(10A149);广州市科技局课题(2008JI-C261)

摘　　要：目的:研究蝙蝠葛碱(Dauricine,Dau)对离体豚鼠气管平滑肌的作用及机制。方法:取豚鼠气管制成气管片,运用离体器官实验的方法,记录蝙蝠葛碱对离体豚鼠气管平滑肌的作用和在不同通道及受体阻滞剂孵育的条件下对豚鼠气管平滑肌的作用的变化。结果:蝙蝠葛碱对离体气管平滑肌有明显的舒张作用,在60～120μmol/L的浓度之间舒张效果具有明显的浓度依赖性(P<0.05)。硝苯地平+Dau组在80～160μmol/L浓度下舒张离体豚鼠气管平滑肌的作用较Dau组弱(P<0.05)。普萘洛尔+Dau组在60～120μmol/L浓度下舒张离体豚鼠气管平滑肌的作用较Dau组弱(P<0.05),在120～160μmol/L浓度下舒张离体豚鼠气管平滑肌的作用与Dau组相近(P>0.05)。结论:蝙蝠葛碱对离体豚鼠气管平滑肌有明显的舒张作用,其机制可能是通过阻断Ca2+通道和激动气管平滑肌上的β2受体有关。AIM：To investigate the relaxation effects of Dauricine（Dau） on isolated tracheal smooth muscles of guinea pigs. METHODS：We made tracheal pieces of guinea pigs,and used experimental methods in isolated organs,recorded the relaxation effect of Dau for isolated tracheal smooth muscles of guinea pigs,and also observed the relaxation effect＇s changes of Dau for isolated tracheal smooth muscles of guinea pigs in different channel and receptor blockers. RESULTS：Dau had a significant relaxing effect on isolated tracheal smooth muscles of guinea pigs,and the relaxation effect had a significant concentration-dependent between 60 to 120 μmol/L concentrations（P0.05）.The relaxation of isolated guinea pig tracheal smooth muscle of nifedipine plus Dau group was weaker than that of Dau group between 80 to 160 μmol/L concentrations（P0.05）.The relaxation of isolated guinea pig tracheal smooth muscle of propranolol plus Dau group was weaker than that of Dau group between 60 to 120 μmol/L concentrations（P0.05）,but the relaxation of isolated guinea pig tracheal smooth muscle of propranolol plus Dau group was similar compared to Dau group between 120 to 160 μmol/L concentrations（P0.05）. CONCLUSION：Dau has a significant relaxing effect on isolated tracheal smooth muscles of guinea pigs and it may be related with β2 receptors and inhibiting calcium channel.

关 键 词：蝙蝠葛碱 离体豚鼠气管平滑肌 CA2+通道 Β2受体 

分 类 号：R285.5[医药卫生—中药学]