青岛文昌鱼(Branchiostoma belcheri tsingtauense)染色体G类带型研究  

STUDIES ON THE G-TYPE CHROMOSOMAL PATTERNS OF AMPHIOXUS BRANCHIOSTOMA BELCHERI TSINGTAUENSE

作　　者：牟洪善[1,2] 王昌留[1] 李金萍[3] 

机构地区：[1]鲁东大学生命科学学院,烟台264025 [2]东华理工大学学团部,南昌330013 [3]东华理工大学信息工程学院,南昌330013

出　　处：《海洋与湖沼》2011年第4期597-602,共6页Oceanologia Et Limnologia Sinica

基　　金：国家"863"项目资助;2008AA092604号;山东省教育厅项目资助;J08LE03号;江西省教育厅项目资助;GJJ11490号

摘　　要：以青岛文昌鱼的晚期囊胚和早期原肠胚的胚胎细胞为实验材料,利用低渗处理、空气干燥法制备染色体玻片标本。在此基础上,为进一步探索显示青岛文昌鱼染色体G带的新方法,采用片龄、蛋白酶K和胰蛋白酶显带技术对青岛文昌鱼的染色体进行了初步研究,显示了青岛文昌鱼染色体的G类带型,绘制了青岛文昌鱼染色体的G类带型模式图,并对三种G类带型作了比较,进而提出了文昌鱼染色体的G带带型与片龄的相互关系。The chromosomal patterns of amphioxus （Branchiostoma belcheri tsingtauens） were studied using blastula and early gastrula stages of embryos underwent hypotonic treatment and air-drying. New techniques for G-type banding were investigated using trypsin, protease K, and slide aging treatment. The G-type bandings of amphioxus were obtained and the chromosomal profiles were shown. The characteristics of the three G-type bands were compared, the similarities and differences were discussed. In addition, the correlation between the slide aging and G-banding was proposed.

关 键 词：青岛文昌鱼 染色体带型 G带 片龄 

分 类 号：Q23[生物学—细胞生物学]