利用ND1、ITS1序列研究三种背角无齿蚌(Anodonta woodiana)分类学问题  

THE SPECIES IDENTIFICATION OF THREE ANODONTA WOODIANA SPECIES BASED ON SEQUENCE DIFFERENCES OF ND1 AND ITS1 GENES

作　　者：赵颖[1,2] 陈修报[1,2] 杨健[1,2] 刘洪波[2] 

机构地区：[1]南京农业大学无锡渔业学院,无锡214081 [2]中国水产科学研究院淡水渔业研究中心中国水产科学研究院内陆渔业生态环境与资源重点开放实验室,无锡214081

出　　处：《海洋与湖沼》2011年第4期619-624,共6页Oceanologia Et Limnologia Sinica

基　　金：江苏省自然科学基金项目;BK2006030号;人力资源和社会保障部留学人才项目;2-115084号;中国水产科学研究院内陆渔业生态环境和资源重点开放实验室开放基金课题;YZ2009-02号;国家自然科学基金项目;31072214号

摘　　要：利用ND1、ITS1序列差异对采自太湖、在形态上被认为是背角无齿蚌、圆背角无齿蚌、椭圆背角无齿蚌的三种个体进行鉴定,并与从NCBI中下载到的同属不同种褶纹冠蚌的ND1、ITS1序列进行对比,分析了基于Kimura 2-parameter模型参数得到的遗传距离,并构建了NJ树。结果表明,本研究中所有背角无齿蚌种间遗传距离变化范围ND1为0—0.0082,ITS1为0.002—0.087,远小于同褶纹冠蚌的遗传距离0.892—0.905和1.1295—1.1307,支持将背角无齿蚌、圆背角无齿蚌、椭圆背角无齿蚌归为同种的观点。Anodonta woodiana （Mollusca, Lamellibranchia, Eulamellibranchia, Unionidae, Anodontinae, Anodonta） is currently used as a sentinel species to monitor environmental contamination for the Freshwater Mussel Watch program （FMWp） in our laboratory. This unionid mussel is broadly distributed in freshwater environment of China and is traditionally divided into 3 species, A. woodiana elliptica, A. woodiana pacifica, and A. woodiana woodiana, based on the differences in shell morphology. However, the morphological features are greatly variable under different environmental conditions and hence taxonomic confusion usually exists when we use this mussel in FMWp. Trying to clarify this problem, we employed the molecular approaches, using the mitochondrial NADH dehydrogenase subunit 1 （ND1） gene and the ribosomal DNA internal transcribed spacer 1 （ITS1）, to re-identify the possible taxonomic characteristics of the mussel individuals that were believed to be different species of A. woodiana, A. pacifica or A. elliptica according to morphological features. Genetic distances were calculated based on the Kimura 2-parameter model, and in turn the neighbor-joining （N J） phylogenetic tree was constructed. For purpose of comparison, the ND1 and ITS1 sequences of Cristaria plicata from NCBI GeneBank were co-analyzed with those of the mussels in this study. The largest intraspecific genetic distances among all studied mussels were 0.087 by the ITS1 gene and 0.0082 by the ND1 gene, which were much lower than those of the interspecific distances. The phylogenetic analysis results of our study suggest that A. woodiana, A. pacifica, and A. elliptica are likely to be the same species.

关 键 词：ND1 ITS1 背角无齿蚌 圆背角无齿蚌 椭圆背角无齿蚌 物种鉴定 

分 类 号：Q959.215[生物学—动物学]