细菌性条斑病菌致病力分化及其对水稻幼苗和成株的致病力差异  

Virulence differentiation of Xanthomonas oryzae pv. oryzicola in rice at seedling and adult stages

作　　者：张荣胜[1,2] 陈志谊[1] 刘永锋[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014 [2]南京农业大学植物保护学院,江苏南京210095

出　　处：《江苏农业学报》2011年第5期996-999,共4页Jiangsu Journal of Agricultural Sciences

基　　金：农业部公益性(农业)行业科研专项(nyhyzx07-056);国家"863"计划现代农业技术领域重大项目(2006AA10A211);江苏省自然科学基金项目(BK2008350)

摘　　要：对来源于湖南、江西、云南、江苏和安徽省的71株细菌性条斑病菌菌株,通过采用苗期注射接种和成株期针刺接种法,分别研究了它们在6个鉴别水稻品种(金刚30、IRBB4、IRBB5、IRBB14、IRBB21和IRBB24)上致病力分化及其对水稻幼苗和成株致病力差异,为品种布局和条斑病菌致病力快速鉴定提供理论依据。试验结果显示,71株条斑病菌菌株在苗期和成株期可分别区分为18个和13个致病型;对苗期的优势致病型为C1,占供试菌株总数的17.0%;成株期的优势致病型为C10,占供试菌株总数的25.4%。研究结果还表明:大多数菌株与水稻品种之间表现为弱互作关系,部分菌株表现为强互作关系。就单个菌株而言,对苗期和成株期的致病力分化是不一致的。整体而言,71株菌株在6个鉴别品种的苗期上可侵染241次,侵染率为56.6%;在成株期上有245次侵染,总侵染率为57.5%。病原菌在不同水稻品种的苗期和成株期上的侵染能力是基本相同的。To provide a theoretical basis for rice cultivar distribution and rapid identification of the virulence of Xanthomonas oryzae pv.oryzicola,the virulence differentiation of 71 isolates of Xanthomonas oryzae pv.oryzicloa collected from Hunan,Jiangxi,Yunnan,Jiangsu and Anhui provinces were identified with six rice cultivars（Jingang30,IRBB4,IRBB5,IRBB14,IRBB21 and IRBB24） containing different resistance genes against bacterial blight by the inoculation methods of leaf-infiltration with needleless syringes at seedling stage and leaf-needling at adult stage.The results showed that the 71 strains were classified into 18 pathotypes at seedling stage and 13 pathotypes at adult stage.The predominant pathotype were C1（17.0% of the total strains） at seedling stage,and C10（25.4% of the total strains） at adult stage.Most isolates had weak interactions with the rice cultivars,and some the isolates had specific interactions.However,for a single strain,the virulence differentiation of Xanthomonas oryzae pv.oryzicloa was not the same in different growth stages.It was also found that all the strains were infected 241 times（the infection rate was 56.6%） and 245 times（the infection rate was 57.5%） at seedling and adult stages,respectively.The virulences of pathogenic bacteria in different rice varieties at seedling and adult stages were basically the same.

关 键 词：水稻条斑病菌 鉴别品种 苗期 成株期 致病力分化 致病型 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]