中药对佐剂性关节炎大鼠中P物质干预作用的研究  

Intervention Effect of Herbs on Substance P in Adjuvant Arthritis Rats

作　　者：戴巧定[1] 宋欣伟[1] 康海英[2] 张彦[1] 

机构地区：[1]浙江中医药大学附属第一医院风湿免疫科,浙江杭州310006 [2]杭州市余杭区第五人民医院,浙江杭州311100

出　　处：《中华中医药学刊》2011年第11期2486-2489,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药青年基金资助项目(2006Y001)

摘　　要：目的:探讨清热化湿、通络止痛方对佐剂性关节炎(AA)大鼠中P物质(SP)的干预作用。方法:以AA大鼠为研究对象,以肿瘤坏死因子(TNF-a)和白介素(IL-1β)作为炎症活动的指标,以雷公藤多甙片为阳性对照药物,造模成功后,给予不同剂量的中药液、雷公藤多甙片混悬液灌胃治疗30d,分别在治疗前后用放射免疫法测定各组大鼠血液、滑膜中的TNF-a、IL-1β、SP以及脊髓后角中的SP水平,以观察中药治疗后各组织中SP的变化。结果:与正常组比较,模型组大鼠血液和滑膜中的SP、TNF-a、IL-1β和脊髓后角中的SP水平明显增高(P<0.05);与模型组比较,给药30d后,各给药组大鼠血液、滑膜中TNF-a、SP及滑膜中IL-1β水平都显著降低(P<0.05);与阳性对照组比较,中药高剂量组能显著降低脊髓后角的SP水平(P<0.05)。结论:该中药方具有抗炎镇痛作用,其作用机理除了降低血清和滑膜中的TNF-a、IL-1β水平外,还可能与降低佐剂性关节炎大鼠外周和中枢的SP水平有关。Objective：To investigate the effects of herbs of clearing heat and resolving dampness and dredging collaterals and relieving pain on substance P（SP） in adjuvant arthritis（AA） rats.Methods：AA rats were randomly divided into the normal group,the model group,the tripterygium polyglucoside group,the low-dose and high-dose herbs groups.TNF-α,IL-1β were used as indicator of inflammatory activity.The tripterygium polyglucoside group was used as the positive control.The treatment groups were respectively intragastric administrated with tripterygium polyglucoside,low-dose and high-dose herbs for 30days after modeling.The levels of TNF-α,IL-1β,SP in blood and synovial membrane and the levels of SP in the spinal dorsal horn were measured by radioimmunoassay before and after treatment.The levels changes of SP in the low-dose and high-dose herbs groups were observed.Results：In the model group,the levels of SP in spinal dorsal horn and TNF-α,IL-1 β,SP in blood and synovial membrane increased compared with the normal group（P0.05）.After treatment with tripterygium polyglucoside and herbs,the degree of right toe swelling in treatment groups were lower than that in model group（P0.05）,and the levels of SP,TNF-α in blood and synovial membrane,IL-1β in synovial membrane were all lower than that in the model group（P0.05）.In addition in the high-dose herbs group the levels of SP in spinal dorsal horn was decreased.Conclution：The herbs in the treatment of adjuvant arthritis rats have favorable anti-inflammatory and analgesic effects that may involved in reducing the levels of the peripheral and central SP besides TNF-α and IL-1β in blood and synovial membrane.

关 键 词：P物质 关节炎 佐剂性 雷公藤 中医药疗法 

分 类 号：R285.5[医药卫生—中药学]