痛风泰颗粒对兔滑膜细胞IL-8和ICAM-1表达的影响  被引量:6

Effects of Tongfengtai Granulars on Rabbit Synovial Cell IL-8 and ICAM-1 Expression

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作  者:张剑勇[1] 刘题章[1] 邱侠[1] 钟力[1] 黄华[1] 

机构地区:[1]深圳市中医院,广东深圳518033

出  处:《中华中医药学刊》2011年第11期2491-2492,共2页Chinese Archives of Traditional Chinese Medicine

基  金:广东省中医药局资助项目(2008022)

摘  要:目的:观察痛风泰颗粒对尿酸钠(MSU)诱导的兔膝滑膜组织中白细胞介素(IL-8)和细胞间黏附分子-1(ICAM-1)表达的影响。方法:取3只新兔分灌生理盐水、痛风泰颗粒溶液、戴芬溶液,1h后抽取静脉血,离心取血清编号为1、2、3,采用改良组织块培养法培养第4代兔膝滑膜细胞,随机分为空白组(NS+血清1)、模型组(MSU+血清1)、中药组(MSU+血清2)、西药组(MSU+血清3)培养3天后,应用ELISA法测定每组滑膜细胞培养液中IL-8和ICAM-1含量。结果:模型组IL-8、ICAM-1的水平明显高于中药组、西药组及空白组,有统计学意义(P<0.05),中药组和西药组之间的IL-8、ICAM-1水平比较无统计学意义(P>0.05)。结论:痛风泰颗粒能够显著抑制尿酸钠诱导的兔滑膜组织IL-8和ICAM-1的表达,表明痛风泰颗粒对痛风的治疗作用可能是通过抑制滑膜组织炎症因子IL-8和ICAM-1的释放来实现的。Objective: To observe Tongfengtai granulars on interleukin(IL-8) and Inter-Cellular Adhesion Molecule-1(ICAM-1) expression in Monosodium Urate(MSU)-induced rabbit knee synovial tissue.Methods: There were three New Zealand rabbits which were lavaged respectively with physiological saline,the Tongfengtai granulars solution and the diclofenac sodium capsule solution,after one hour,centrifugal venou' serum from three New Zealand rabbits were collected and named respectively as serum 1,serum 2 and serum 3;we cultivated rabbits'knee synovial cells to 3 or 4 generations through the improved anization cultivate method and randomly divided into three groups.For example,blank groups that mixed with physiological saline and serum1,model groups that mixed with urate and serum1,Chinese traditional medicine groups that mixed with urate and serum2,western medicine groups that mixed with urate and serum3;were trained for three days,contents of each group of synovial cells about IL-8 and ICAM-1 were determined through ELISA.Results: The model groups' contents about IL-8、ICAM-1were highest than other groups obviously(P0.05),but the outcomes about blank groups compaing with the Western medicine groups or the Chinese traditional medicine groups'contents of IL-8、ICAM-1were no statistical significance(P0.05).Conclusion: The Tongfengtai granulars and the diclofenac sodium capsule could significantly inhibit the uric acid sodium-induced rabbit synovial tissue IL-8,ICAM-1 expression,indicating that Tongfengtai granulars treatment of gout may be inhibiting the release of inflammatory cytokines as IL-8 and ICAM-1 in the synovial tissue.

关 键 词:痛风泰颗粒 痛风 滑膜细胞培养 IL-8 ICAM-1 

分 类 号:R285.5[医药卫生—中药学]

 

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