不同剂量嗅鞘细胞蛛网膜下腔移植修复脊髓损伤的实验研究  

Different doses of olfactory ensheathing cells transplantation in subarachnoid for repairment of spinal cord injury,an experimental study

作　　者：李军[1] 魏开斌[1] 刘红[2] 刘雨亮[1] 王永红[1] 张文正[1] 卓锋[1] 王强[1] 

机构地区：[1]泰安市中心医院骨一科,泰安市271000 [2]泰安市中心医院护理部,泰安市271000

出　　处：《中国脊柱脊髓杂志》2011年第11期929-933,共5页Chinese Journal of Spine and Spinal Cord

摘　　要：目的:探讨嗅鞘细胞蛛网膜下腔移植治疗脊髓损伤的可行性、安全性及细胞移植数量与疗效的关系。方法:应用改良Allen′s法制备大鼠T10脊髓损伤模型,随机分四组:大剂量嗅鞘细胞蛛网膜下腔移植组(A组,2×106个)、中剂量嗅鞘细胞蛛网膜下腔移植组(B组,2×105个)、小剂量嗅鞘细胞蛛网膜下腔移植组(C组,2×104个)、DMEM/F12培养液蛛网膜下腔移植组(D组)。术后即刻进行细胞移植,术后第4、10周行CBS功能评分及组织学检查,观察脊髓神经功能修复情况及损伤区神经纤维数量。结果:CBS功能评分示A、B组明显好于C、D两组(P<0.05);A、B两组间比较无差异,C、D两组间比较无差异(P>0.05)。组织学观察发现A、B组神经纤维数量多于C、D组。结论:急性期嗅鞘细胞经蛛网膜下腔移植治疗脊髓损伤安全有效,急性期移植细胞数量较大则脊髓神经功能恢复较好。Objective：To investigate the feasibility, safety and dose-effect relationship of olfactory ensheathing cell transplantation for spinal cord injury.Method：Rats with T10 spinal cord injury induced by modified Allen method were randomly divided into four groups and received immediate （：ell transplantation as followings： high-dose olfactory ensheathing cells （OECs） transplantation in subarachnoid （group A）,medium-dose trans- plantation （group B）,low-dose transplantation （group C） ,control group with DMEM/F12 transplantation （group D）.The neurofnnctional recovery was noted by combined behavioral scores （CBS）;the axons regeneration and the survival of olfactory ensheathing cells at the different time-point recorded either.Results：The neurofunction of group A and B improved significantly compared with group C or D according to combined behavioral scores（CBS）,whereas no difference was noted between group A and B,as well as same as between group C and D.It was found by histological observation that the number of nerve fibers of group A and group B was more than group C and D.Conclusion：The OEC transplantation in subarachnoid for acute spinal cord injury is reliable, and high dose shows good prognosis.

关 键 词：嗅鞘细胞 脊髓损伤 蛛网膜下腔移植 移植剂量 

分 类 号：R683.2[医药卫生—骨科学]