猪FBXL8基因第二内含子的克隆测序及SNP位点检测  

Cloning,Sequencing and SNP Site Detection of the Second Intron of Porcine FBXL8 Gene

作　　者：李勇[1,2,3] 杨述林[3] 崔文涛[3] 牟玉莲[3] 唐中林[3] 储明星[3] 彭克美[2] 李奎[3] 

机构地区：[1]江西农业大学动物科技学院,江西南昌330045 [2]华中农业大学动物科学-动物医学院,湖北武汉430070 [3]中国农业科学院畜牧研究所家养动物遗传资源与种质创新重点开放实验室,北京100193

出　　处：《江西农业大学学报》2011年第5期938-941,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30571330;30700569);江西省科技支撑计划项目和江西省农业厅项目

摘　　要：为探明猪FBXL8基因的分子结构特征,本试验以人FBXL8基因的mRNA序列作为信息探针,搜集猪同源基因EST序列,并构建EST重叠群Contig,设计出合适的引物,以湖北通城、大白和长白猪基因组为模板,应用PCR技术克隆测定FBXL8基因的第二内含子序列。序列分析结果表明猪FBXL8基因第二内含子为855 bp。通过运用SEQMAN软件对3个品种的序列比较分析,初步发现第二内含子的第325、361和493位碱基为SNP位点。In order to explore the molecular structural feature of porcine FBXL8 gene,the Homo FBXL8 mRNA sequence was used as an information probe to search for the porcine homologous ESTs sequence in this experiment,and the ESTs contig was constructed.Then the primers were designed according to it.Tongchen,Landrace and large white breeds DNA were used as the genomic template to amplify the second intron of porcine FBXL8 gene by PCR technique.The sequence analysis result indicates that it is a sequence of 855 bp long.Moreover,SEQMAN software was used to compare and analyze these sequences,and it was preliminarily found that there are three SNPs in the 325th,361th and 493th site respectively.

关 键 词：FBXL8 第二内含子 克隆测序 SNP位点检测 

分 类 号：S828[农业科学—畜牧学]