罗非鱼创伤弧菌的分离鉴定和药敏试验  

Isolation and Identification of Vibrio vulnificus from Infected Tilapia and Its Drug-sensitivity Analysis

作　　者：黎炯[1,2] 叶星[1,2] 卢迈新[1,2] 邓国成[1] 高风英[1] 可小丽[1] 朱华平[1] 黄樟翰[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所,广东广州510380 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《江西农业大学学报》2011年第5期965-970,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家863专项(2011AA100404);现代农业产业技术体系专项资金资助(CARS-49);广东省农业攻关项目(2009B020201003);广东省海洋渔业科技推广专项(A201001C05)资金资助

摘　　要：2010年春季广东、海南的养殖罗非鱼苗种出现较大范围的死亡现象,在广东珠海某罗非鱼养殖场的发病罗非鱼体上分离到一株病原菌ZH1。人工感染试验显示该分离菌株具有较强毒力,该病原菌经ATB 32E细菌鉴定系统鉴定和16S rRNA基因序列分析,确定为创伤弧菌(Vibrio vulnificus)。药敏试验结果显示,该菌株对诺氟沙星、头孢克洛、氧氟沙星和壮观霉素等19种试验药物敏感。本研究病原的鉴定与药物敏感性试验结果为罗非鱼病害的有效防控提供参考。In Spring of 2010,large-ranging diseases occurred in tilapia farms in Guangdong and Hainan provinces.A Gram-negative pathogenic bacterial strain ZH1 was isolated from diseased tilapia fry cultured in a farm in Zhuhai,Guangdong.Artificial infection experiments showed that the isolated strain possessed strong virulence.This isolated strain was identified as Vibrio vulnificus using ATB 32E identification and 16S rRNA sequence analysis.The drug-sensitivity test of a total of 29 antimicrobial agents showed that the strain ZH1 was highly sensitive to 18 agents such as Norfloxacin,Cefaclor,Ofloxacin and Spectinomycin.The results of pathogen identification and drug-sensitivity test will be helpful for effective prevention of fish diseases.

关 键 词：罗非鱼 创伤弧菌 分离 鉴定 药物 敏感性 

分 类 号：S965.125[农业科学—水产养殖]