HPLC-ELSD法测定利肝隆颗粒中黄芪甲苷的含量  

Content Determination of Astragaloside in Liganlong Granules by HPLC-ELSD

作　　者：王红霞 王蕾 

机构地区：[1]江苏盐城市第一人民医院,盐城市224001 [2]江苏盐城市药品检验所,盐城市224002

出　　处：《中国药房》2011年第44期4208-4209,共2页China Pharmacy

摘　　要：目的:建立测定利肝隆颗粒中黄芪甲苷含量的方法。方法:采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法。色谱柱为Agilent C18柱,流动相为乙腈-水(32∶68),流速为1.0mL·min-1,进样量为20μL,柱温为35℃;ELSD检测器漂移管温度为100℃,载气流量为2.7L·min-1。结果:黄芪甲苷进样量在0.3867～3.8670μg范围内与峰面积常用对数的积分值线性关系良好(r=0.9992);加样回收率为99.0%、99.0%、98.7%,RSD=0.6%(n=9)。结论:本方法操作简便、准确、灵敏度高、重复性好,可作为利肝隆颗粒的质量控制方法。OBJECTIVE： To establish the method for the content determination of astragaloside in Liganlong granules. METHODS： HPLC-ELSD method was adopted. The determination was performed on Agilent C18 column with mobile phase consisted of acetonitrile-water （32 ∶ 68） at the flow rate of 1.0 mL·min^-1. The injection volume was 20 μL and column temperature was 35 ℃. The drift tube temperature was 100 ℃ and the flow rate of carrier gas was 2.7 L·min^-1. RESULTS： The linear range of astragaloside was 0.386 7～3.867 0 μg （r=0.999 2）. Average recoveries were was 99.0%, 99.0% and 98.7% （RSD=0.6%, n=9）. CONCLUSION： The method is simple and accurate, and has good sensitivity and reproducibility. It could be used for quality control of Liganlong granules.

关 键 词：利肝隆 黄芪甲苷 含量 高效液相色谱-蒸发光散射检测器法 

分 类 号：R917[医药卫生—药物分析学]