阳离子交换纤维微柱离心法测定盐酸表阿霉素脂质体包封率  

Determination of entrapment efficiency of epirubicin hydrochloride liposome using cation exchange fiber minicolumn centrifugation

作　　者：杨强[1] 张岩柏[2] 牟丽娜[3] 李哲[1] 邓意辉[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]武警沈阳指挥学院门诊部,辽宁沈阳110113 [3]辽源市中医院,吉林辽源136200

出　　处：《沈阳药科大学学报》2011年第11期853-856,932,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立盐酸表阿霉素脂质体包封率的测定方法。方法以改良乙醇注入-pH梯度法制备盐酸表阿霉素脂质体,以阳离子交换纤维装柱,离心分离脂质体和游离药物,采用可见分光光度法测定药物含量,计算包封率。结果盐酸表阿霉素在5.0～35.0 mg.L-1内线性关系良好(r=0.9998),柱长为1 cm的阳离子交换纤维柱可以完全吸附0.1 mL质量浓度为0.5～2.0 g.L-1的盐酸表阿霉素溶液,且脂质体柱回收率大于99.0%。结论阳离子交换纤维微柱离心法可用来测定盐酸表阿霉素脂质体包封率。Objective To establish a method for the determination of entrapment efficiency of epirubicin hydrochloride liposome. Methods The epirubicin hydrochloride liposome was prepared by modified ethanol injection and pH gradient method. Cation exchange fiber was employed to separate the free drug from the liposome. The concentration of epirubicin hydrochloride was determined by the visible spectrophotometry. Results The visible spectrophotometry method had good linearity in the range of 5.0-35.0 mg. L-1（ r = 0. 999 8 ）. The free epirubicin hydrochlofide （0. 5- 2. 0 g. L -1 ） was well adsorbed by cation exchange fiber with length of 1 cm, and the recovery of liposome was beyond 99.0%. Conclusions This method can be used for determinating the entrapment efficiency of epirubicin hydrochloride liposome.

关 键 词：盐酸表阿霉素 脂质体 阳离子交换纤维 包封率 

分 类 号：R94[医药卫生—药剂学]