胃旁路术对GK大鼠糖代谢影响及其与Ghrelin的关系  

Gastric bypass surgery improves glucose metabolism possibly by decreasing ghrelin levels in Goto-Kakizaki rats

作　　者：曹超[1] 曾荣[2] 张鹏[1] 周晓磊[1] 尤胜义[1] 

机构地区：[1]天津医科大学总医院普通外科,天津市300052 [2]天津市南开医院,天津市300100

出　　处：《世界华人消化杂志》2011年第26期2768-2771,共4页World Chinese Journal of Digestology

摘　　要：目的:观察胃旁路术(gastric bypass,GBP)对非肥胖型2型糖尿病大鼠(Goto-Kakizaki rats;GK大鼠)血糖的影响,并探讨其机制.方法:GK大鼠20只,Wistar大鼠10只,均为雄性.分为GK手术组、GK假手术组和Wistar假手术组,每组10只.手术组行胃旁路术.测定术前1wk及术后第1、2、4、8、12wk各组大鼠的空腹血糖(FPG)、糖化血红蛋白(HbAlc)和血清胰岛素(INS),并同期检测血清Ghrelin水平.结果:术后第12周,GK手术组大鼠FPG和HbAlc分别由术前的11.36mmol/L±1.14mmol/L和8.91%±0.36%下降到8.36±0.62mmol/L和6.35%±0.46%,血清INS由术前32.76mIU/L±2.37mIU/L上升到55.14mIU/L±5.45mIU/L,而Ghrelin由术前928.53ng/L±58.66ng/L下降到367.83ng/L±27.78ng/L,差异均有统计学意义(P<0.05).结论:GBP可以明显改善GK大鼠的糖代谢,其作用机制可能与降低Ghrelin水平,进而促进胰岛素分泌有关.AIM： To investigate the influence of gastric bypass （GBP） surgery on glucose metabolism in Goto-Kakizaki （GK） rats and to explore the possible mechanisms involved. METHODS： Twenty male GK rats and 10 male Wistar rats were randomized into three groups： GK operation group, GK sham operation group and Wistar sham operation group. The GK operation group underwent gastric bypass sur- gery. The levels of fasting plasma glucose （FPG）, glycosylated hemoglobin （HbAlc）, serum insulin （INS） and ghrelin were monitored I week before surgery and 1, 2, 4, 8 and 12 weeks after surgery. RESULTS： In the GK operation group, FPG level decreased from （11.36 ± 1.14） mmol/L be-fore surgery to （8.36 ± 0.62） mmol/L 12 weeks after surgery, and HbAlc from （8.91 ± 0.36）% to （6.35±0.46）%. Serum INS increased from （32.70 ± 2.37） mIU/L before surgery to （55.14 ± 5.45） mIU/L 12 weeks after surgery, while serum ghrelin level decreased from （928.53 ±58.66） pg/mL to （367.83 ± 27.78） pg/mL. All the above parameters differed significantly between before surgery and 12 weeks after surgery （all P 〈 0.05）. CONCLUSION： GBP can significantly improve glycometabolism in GK rats possibly by decreasing ghrelin levels and promoting insulin secretion.

关 键 词：2型糖尿病 胃旁路术 GK大鼠 糖代谢 GHRELIN 

分 类 号：R656.6[医药卫生—外科学]