阳离子脂质体乳剂增强防龋疫苗黏膜免疫效果的研究  

Preparation of Cationic Lipid Emulsion-encapsulated Anti-caries Vaccines and its Effect of Enhancing the Mucosal Immune

作　　者：杨亚萍[1,2] 刘明谆[1] 闵智鹏[1] 樊明文[1] 李宇红[1] 

机构地区：[1]武汉大学口腔医学院口腔生物医学教育部重点实验室,湖北武汉430079 [2]南京大学口腔医学院.南京市口腔医院牙体牙髓病科

出　　处：《口腔医学研究》2011年第10期849-852,共4页Journal of Oral Science Research

基　　金：国家自然基金资助项目(编号:80);武汉市发展引导类计划资助项目(编号:201060938367)

摘　　要：目的:观察阳离子脂质体增强防龋疫苗诱导的小鼠特异性IgG和唾液IgA抗体水平。方法:以阳离子脂质体乳剂分别包被防龋DNA疫苗(pGJA-P/VAX)和基因重组rPAc蛋白疫苗,用DNA初免,2周后蛋白加强免疫的策略滴鼻免疫6～8周龄的BALB/c小鼠,分别收集初免后0、2、4、6、8、10、12、16周的血清和唾液,用酶联免疫吸附实验(ELISA)检测血清和唾液中特异性抗体的水平。结果:制备的乳剂疫苗CLE/DNA和CLE/rPAc的粒径分别为134.6nm和230nm,ZETA电位为36mV和18.5mV,乳剂疫苗免疫小鼠后可以诱导血清特异IgG和唾液特异IgA产生,并且实验组(LE/DNA初免+LE/rPAc加强免疫组)能产生较对照组(DNA初免+rPAc加强免疫组)更持久的黏膜免疫反应,两组比较差异有显著性(P<0.05)。结论:阳离子脂质体乳剂是有效的基因疫苗载体,与防龋疫苗(DNA和rPAc蛋白)合用免疫小鼠,可增强血清特异性IgG和唾液特异性IgA抗体水平,延长抗体维持时间,具有较好黏膜免疫反应增强作用。Objective： To observe the enhancement of specific IgG in serum and salivary IgA in mice after immunization by anti-caries vaccine in cationic liposome emulsion.Methods： The anti-caries gene vaccines（pGJA-P/VAX and rPAc） were coated with the cationic liposomes emulsion,forming CLE/DNA and CLE/rPAc.The strategy of DNA prime-rPAc boost was intranasal immunized 6-8 weeks old BALB/c mice.At 0w,2w,4w,6w,8w,10w,12w and 16w after the initial immunization,we collected the serum and saliva of mice,and observed the levels of specific antibodies in serum and saliva by enzyme-linked immunosorbent assay（ELISA）.Results： Cationic liposome emulsion（CLE） was coated with DNA and rPAc protein.We obtained the complexes： CLE/DNA and CLE / rPAc.The mean particle size of the complexes were 134.6nm and 230nm.ZETA potential were 36MV and 18.5MV.Both the experimental group（CLE/DNA prime ＋ CLE / rPAc boost） and the control group（DNA prime ＋ rPAc boost） after the initial immunization could be induced the specific IgG production in the mouse serum and specific IgA in saliva.The experimental group had more lasting mucosal immune response than the control group,and the experimental group was significantly different from the control group（P less than 0.05）.Conclusion： The cationic liposome emulsion is an effective mucosal vector,and co-immunized mice with the anti-caries vaccines（DNA and rPAc protein） can induce the production of specific IgG and IgA antibody in mice,and can induce more durable mucosal immunity reaction than the control group.

关 键 词：防龋疫苗 阳离子脂质体乳剂 黏膜免疫 

分 类 号：R392.1[医药卫生—免疫学]